製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
IHC-Fr Use at an assay dependent concentration. PubMed: 19553662Use at an assay dependent dilution (PMID 19553662).
WB 1/500. Can be blocked with Mouse Angiopoietin 2 peptide (ab9077). There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong.

ターゲット情報

  • 機能
    Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 配列類似性
    Contains 1 fibrinogen C-terminal domain.
  • ドメイン
    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

画像

  • This image is courtesy of Marion Scharpfenecker 2002

    Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.

    Lane 1 - mock
    Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
    Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
    Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
    Lane 5 - wt

     

  • ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of murine brain coronal sections, staining Angiopoietin 2 with ab8452 at 1/500 dilution. Sections were taken from control mice (left) or mice subjected to middle cerebral artery occlusion (right).

参考文献

This product has been referenced in:
  • Bohn KA  et al. Inhibition of VEGF and Angiopoietin-2 to Reduce Brain Metastases of Breast Cancer Burden. Front Pharmacol 8:193 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28443023) »
  • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . Read more (PubMed: 28490763) »

See all 17 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Umbilical Vein Endothelial Cell)
Permeabilization
Yes - 0.5% Triton X-100 for 20min
Specification
Human Umbilical Vein Endothelial Cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
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投稿 Aug 23 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
Yes - 0.5% Triton X100
Specification
mouse kidney
Blocking step
2.5% BSA / 2.5% donkey serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 03 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Treatment
OVA I.P, I.N
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 05 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10%gel)
Sample
Mouse Tissue lysate - whole (lung)
Specification
lung
Treatment
OVA-induce
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 38°C
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投稿 Dec 03 2012

Thank you very much for your reply.

I will forward your request to my colleagues in our Hong Kong office, and someone will be in touch with you promptly.

Have a nice day!

Thank you very much for your valuable review.
I'm writing to follow-up with you, as the results were below average and we do guarantee this antibody to work in Western blot with mouse samples. I'm sorry to see that there were several non-specific ...

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Thank you for your enquiry. The Tie2 precipitation experiment was performed by an outside collaborator, and we do not have any further information at hand regarding this. I have tried to email the collaborator to see if we can obtain this infor...

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Thank you for your enquiry. ab8451 is specific for mouse Angiopoietin 1. ab8452 cross-reacts with human (reacts with Ang-2 and weakly with Ang-1) and mouse (specific for mAng-2). There is little sequence homology between the two epitopes of 1 vers...

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All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1. Western blot analysis showed tha...

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This antibody has never been tested in IHC on mouse tissue sections, therefore we can't guarantee results.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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