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Our Abpromise guarantee covers the use of ab8452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19553662Use at an assay dependent dilution (PMID 19553662).|
|WB||1/500. Can be blocked with Mouse Angiopoietin 2 peptide (ab9077). There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong.|
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.
Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt