Anti-Angiopoietin 1 抗体 (ab8451)
Key features and details
- Rabbit polyclonal to Angiopoietin 1
- Suitable for: ICC/IF, IHC-P, WB, ELISA
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Angiopoietin 1 antibody
Angiopoietin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Angiopoietin 1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, ELISAmore details -
種交差性
交差種: Mouse, Human, Pig -
免疫原
Synthetic peptide corresponding to Mouse Angiopoietin 1 aa 21-40 (N terminal).
Sequence:CNQRRNPENGGRRYNRIQHGQ
Database link: O08538 -
特記事項
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8451の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
1/500. Detects a band of approximately 57 kDa. Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/500. Detects a band of approximately 57 kDa. Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
翻訳後修飾
Glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 284 Human
- Entrez Gene: 11600 Mouse
- Entrez Gene: 397009 Pig
- Omim: 601667 Human
- SwissProt: Q15389 Human
- SwissProt: O08538 Mouse
- SwissProt: Q9BDY8 Pig
- Unigene: 369675 Human
see all -
別名
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
画像
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Supernatants of mouse-angiopoietin-expressing endothelial cells.
Lane 1 - wt endothelial cell
Lane 2 - mouse Ang-1 (clone 1-8) expressing cells
Lane 3 - mouse Ang-1 (clone 1-15) expressing cells
Lane 4 - mouse Ang-2 (clone 2-9) expressing cells -
ab8451 was diluted 1:500 to detect Angiopoietin 1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (53)
ab8451 は 53 報の論文で使用されています。
- Klomjit N et al. Microvascular remodeling and altered angiogenic signaling in human kidneys distal to occlusive atherosclerotic renal artery stenosis. Nephrol Dial Transplant 37:1844-1856 (2022). PubMed: 35451482
- Yu L et al. Intrarenal Single-Cell Sequencing of Hepatitis B Virus Associated Membranous Nephropathy. Front Med (Lausanne) 9:869284 (2022). PubMed: 35935760
- Smeda M et al. Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged mice. Front Mol Biosci 9:1050112 (2022). PubMed: 36504711
- Huang W et al. c-Myc-induced circ-NOTCH1 promotes aggressive phenotypes of nasopharyngeal carcinoma cells by regulating the miR-34c-5p/c-Myc axis. Cell Biol Int 45:1436-1447 (2021). PubMed: 33675278
- Luck R et al. The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner. Cell Rep 36:109522 (2021). PubMed: 34407407