製品の概要

  • 製品名Anti-Angiomotin antibody
    Angiomotin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Angiomotin
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (TANKQLAEKE YEGSEDTRKT ISQLFAKNKE SQREKEKLEA ELATARSTNE) of human AMOT (NP_573572).

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
  • 組織特異性Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
  • 配列類似性Belongs to the angiomotin family.
  • ドメインThe coiled coil domain interacts directly with the BAR domain of ARHGAP17.
    The angiostatin binding domain (871-1005) allows the binding to angiostatin.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AMOT antibody
    • AMOT_HUMAN antibody
    • Angiomotin antibody
    • Angiomotin p130 isoform antibody
    • KIAA1071 antibody
    see all

Anti-Angiomotin antibody 画像

  • Anti-Angiomotin antibody (ab83381) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 72 kDa

Anti-Angiomotin antibody (ab83381) 使用論文

ab83381 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"