製品の概要
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製品名Anti-Angiomotin antibody
Angiomotin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Angiomotin
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由来種Rabbit
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アプリケーション適用あり: ELISA, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
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免疫原
Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (TANKQLAEKE YEGSEDTRKT ISQLFAKNKE SQREKEKLEA ELATARSTNE) of human AMOT (NP_573572).
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ポジティブ･コントロール
- 293T cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab83381 is purified by peptide affinity chromatography method.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab83381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay, 1:1562500. |
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| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
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組織特異性Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
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配列類似性Belongs to the angiomotin family.
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ドメインThe coiled coil domain interacts directly with the BAR domain of ARHGAP17.
The angiostatin binding domain (871-1005) allows the binding to angiostatin. -
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
- Information by UniProt
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参照データベース
- Entrez Gene: 422336 Chicken
- Entrez Gene: 100054069 Horse
- Entrez Gene: 154796 Human
- Entrez Gene: 27494 Mouse
- Entrez Gene: 300289 Rat
- Omim: 300410 Human
- SwissProt: Q4VCS5 Human
- SwissProt: Q8VHG2 Mouse
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別名
- AMOT antibody
- AMOT_HUMAN antibody
- Angiomotin antibody
see all
画像
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Western blot - Angiomotin antibody (ab83381)Anti-Angiomotin antibody (ab83381) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
プロトコール
参考文献
ab83381 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"