Anti-Angiomotin 抗体 (ab117776)
Key features and details
- Rabbit polyclonal to Angiomotin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Angiomotin antibody
Angiomotin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Angiomotin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1034-1084 of Human Angiomotin (NP_001106962.1).
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ポジティブ・コントロール
- 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab117776 was affinity purified using an epitope specific to Angiomotin immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 118 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 118 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. -
組織特異性
Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta. -
配列類似性
Belongs to the angiomotin family. -
ドメイン
The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
The angiostatin binding domain (871-1005) allows the binding to angiostatin. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein. - Information by UniProt
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参照データベース
- Entrez Gene: 465814 Chimpanzee
- Entrez Gene: 100054069 Horse
- Entrez Gene: 154796 Human
- Entrez Gene: 27494 Mouse
- Entrez Gene: 300289 Rat
- Entrez Gene: 707701 Rhesus monkey
- Omim: 300410 Human
- SwissProt: Q4VCS5 Human
see all -
別名
- AMOT antibody
- AMOT_HUMAN antibody
- Angiomotin antibody
see all
画像
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All lanes : Anti-Angiomotin antibody (ab117776) at 0.04 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 3 seconds -
Detection of Angiomotin by Western Blot of Immunprecipitate.
ab117776 at 1µg/ml staining Angiomotin in 293T whole cell lysate immunoprecipitated using ab117776 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab117776 は 1 報の論文で使用されています。
- Nakajima H et al. Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance. Dev Cell 40:523-536.e6 (2017). PubMed: 28350986