製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 14.4 kDa. Non-reducing conditions and overnight incubation is recommended. Reducing conditions can be used but often needs higher amounts of sample.
ICC/IF 1/500.
IHC-P 1/500.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
  • 組織特異性Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
  • 関連疾患Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy.
  • 配列類似性Belongs to the pancreatic ribonuclease family.
  • 発生段階Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALS9 antibody
    • AMYOTROPHIC LATERAL SCLEROSIS antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG I antibody
    • ANG1 antibody
    • ANGI antibody
    • ANGI_HUMAN antibody
    • Angiogenin antibody
    • Angiogenin ribonuclease RNase A family, 5 antibody
    • Epididymis luminal protein 168 antibody
    • HEL168 antibody
    • MGC22466 antibody
    • MGC71966 antibody
    • Ribonuclease 5 antibody
    • Ribonuclease RNase A Family 5 antibody
    • RNase 5 antibody
    • RNASE4 antibody
    • RNASE5 antibody
    see all

Anti-Angiogenin antibody [14017.7] 画像

  • ab10600 staining Angiogenin in murine dermis (including follicle) by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 10% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An Alexa Fluor®555 (red) conjugated donkey anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

    See Abreview

  • Anti-Angiogenin antibody [14017.7] (ab10600) at 2 µg/ml + Recombinant human Angiogenin (E. coli derived) at 1 µg

    Secondary
    Anti-mouse IgG conjugated to HRP at 1/5000 dilution
    Developed using the ECL technique

    Exposure time : 45 minutes

    Image courtesy of an anonymous Abreview.

Anti-Angiogenin antibody [14017.7] (ab10600) 使用論文

This product has been referenced in:
  • Blanco S  et al. Aberrant methylation of tRNAs links cellular stress to neuro-developmental disorders. EMBO J 33:2020-39 (2014). Mouse, Human . Read more (PubMed: 25063673) »
  • Padhi AK  et al. Computational and functional characterization of Angiogenin mutations, and correlation with amyotrophic lateral sclerosis. PLoS One 9:e111963 (2014). ICC/IF ; Human . Read more (PubMed: 25372031) »

See all 5 Publications for this product

Product Wall

Thanks for your email. I agree that your blot looks much better. Great idea to change the secondary antibody! It may be that this higher than expected band is non-reduced Angiogenin. I suggest running a reduced and non-reduced sample of each cell line....

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The liver sample extract ab29889 was used as a sample for western blotting where Angiogenin was detected by another anti-Angiogenin antibody, ab95389. This western blot is shown on the datasheet for ab95389 http://www.abcam.com/Angiogenin-antibody-ab95...

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Thank you for your enquiry. I am happy to help you troubleshoot your western blotting experiment. I just have a few additional questions: What is protein sample? (what cell line or tissue and from what species) Have you stained these samples with any o...

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Thank you for your reply and for providing this protocol information.  We will use this to investigate this issue further. Did you purchase the antibody directly from Abcam or from one of our distributors?  I have not been able to find the order nu...

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Thank you for contacting Abcam regarding ab10600. I am sorry that you have been experiencing difficulties with this antibody in IHC.  This product is guaranteed by our Abpromise for IHC-Fr in mouse tissue.  For our records, I would like to gather s...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Dermis (including follicle))
Specification Dermis (including follicle)
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C
Username

Abcam user community

Verified customer

投稿 Jan 25 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mrs. Cindi Roberts

Verified customer

投稿 Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Recombinant Human Angiogenin (E. coli derived))
Loading amount 1 µg
Specification Recombinant Human Angiogenin (E. coli derived)
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"