製品の概要

  • 製品名
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Control Sera 60 <=10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Control Sera 30 <=9.5%
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプ
    Competitive
  • 検出感度
    = 0.01 ng/ml
  • 検出範囲
    0.1 ng/ml - 10 ng/ml
  • 添加回収試験

    100.91 %

  • ステップ
    Multiple steps standard assay
  • 製品の概要

    Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.

     

    A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Washing Solution 1 x 50ml
    Androstenedione Control 1 x 1ml
    Androstenedione Standard 0 – 0 ng/mL 1 x 1ml
    Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml
    Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml
    Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml
    Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml
    Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml
    Androstenedione-HRP Conjugate 1 x 21ml
    Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit
    Cover foils 1 unit
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 別名
    • 4 androstenedione

アプリケーション

Our Abpromise guarantee covers the use of ab108672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

画像

  • Representative Standard Curve using ab108672

プロトコール

参考文献

ab108672 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us and your interest in our products.

The Androstenedione Human ELISA Kit (ab108672) is provided with androstenedione standard which allows you to perform a standard curve to assess that the kit is working as expected...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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