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Our Abpromise guarantee covers the use of ab15272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 21163940|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 87 kDa. ab15272 reacts strongly with some immature isoforms and cleavage products (Range 30-70 kDa). Weaker signal from mature isoforms (over 100 kDa) (this maybe due to steric inhibition by post-translational modifications).|
This image is courtesy of an anonymous Abreview
ab15272 at a 1/100 dilution staining Amyloid Precursor protein from human HEK cells by Immunocytochemistry. The antibody was incubated with the cells for 12 hours and then detected using a goat anti-rabbit IgG Alexa-Fluor ® 568.
ab15272 at a 1/100 dilution staining Amyloid Precursor Protein from Rat brain tissue by Immunocytochemistry. The antibody was incubated with the cells for 18 hours at 4°C and then detected using a goat anti-rabbit secondary Alexa-Fluor® 594.
ab15272 at a 1/100 dilution staining Amyloid Precursor Protein from Rat Cells (Hippocampal neuron culture) by Immunocytochemistry. The antibody was incubated with the cells for 12 hours and then detected using a goat anti-rabbit IgG Alexa-Fluor® 568.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Alzheimers brain tissue labelling Amylois Precursor Protein with ab15272.
ab15272 staining Amyloid Precursor Protein in 15 month old TASTPM mouse brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in 10mM citric acid buffer (pH 6.0) and blocking in 1% BSA for 10 minutes at 25°C. The primary antibody was used at 1/300 dilution, and incubated with sample for 2 hours. A Biotin conjugated goat polyclonal to rabbit IgG at 1/200 dilution, was used as secondary.
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