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Synthetic peptide corresponding to Human Amyloid Precursor Protein aa 90-120 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab12271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 116 kDa. The immunogen region (90-120) appears to refold and form non-native disulphide bonds after the protein has been transferred to nitrocellulose and left in biological buffer for longer than 24 hrs before applying ab12271. This refolding prevents ab12271 from accessing its epitope. Hence it is important to reduce and alkylate samples prior to SDS-PAGE and immunoblotting. It is possible to reduce and alkylate the proteins after transfer to nitrocellulose and before immunoblotting with ab12271.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|