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Synthetic peptide corresponding to Human Amyloid Precursor Protein (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab12269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 116 kDa.|
|ICC||1/100 - 1/500. Requires denatured and reduced sample to allow access to epitope.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Requires denatured and reduced sample to allow access to epitope.|
Soluble extract of macaque brain (Macaca fasicularis, lanes 1, 3, 5) or conditioned medium from CHO cells expressing recombinant human APP770 (lanes 2, 4, 6) were separated by 7.5% SDS-PAGE, transferred to nitrocellulose and immunoblotted with the following antibodies:
Interpretation of Legend: DE2B4 recognises an epitope present in soluble APP cleaved by