Rabbit polyclonal to Amylin
Rabbit, Cat, Dog, Baboon
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Amylin.
This antibody gave a positive signal in a human pancreas tissue section.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
Belongs to the calcitonin family.
The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
Amyloid fibrils are degraded by IDE.
Information by UniProt
Diabetes associated peptide antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin antibody (ab77387)
IHC image of Amylin staining in Human Pancreas section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77387, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
has not yet been referenced specifically in any publications.
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