製品の概要

  • 製品名Anti-AMSH antibody
    AMSH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AMSH
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSDHGDVSLP PEDRVRALSQ LGSAVEVNED IPPRRYFRSG VEIIRMASIY) of Human AMSH (NP_998787).

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • ドメインThe JAMM motif is essential for the protease activity.
  • 翻訳後修飾Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.
  • 細胞内局在Nucleus. Membrane. Cytoplasm. Early endosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Anti-AMSH antibody 画像

  • Anti-AMSH antibody (ab81217) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-AMSH antibody (ab81217) 使用論文

ab81217 has not yet been referenced specifically in any publications.

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