Anti-AMPK gamma 3 抗体 (ab38228)


  • 製品名Anti-AMPK gamma 3 antibody
    AMPK gamma 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AMPK gamma 3
  • 特異性This antibody is specific for PRKAG3.
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the sequence of human PRKAG3 within aa 100-200.

  • ポジティブ・コントロール
    • Breast carcinoma tissue and mouse brain tissue lysate.



Our Abpromise guarantee covers the use of ab38228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 51 kDa).


  • 機能AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
  • 組織特異性Skeletal muscle, with weak expression in heart and pancreas.
  • 配列類似性Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
    Contains 4 CBS domains.
  • ドメインThe AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
    The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
  • 翻訳後修飾Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 AMP activated protein kinase subunit gamma 3 antibody
    • 5''-AMP-activated protein kinase subunit gamma-3 antibody
    • AAKG3_HUMAN antibody
    • AMPK gamma 3 chain antibody
    • AMPK gamma3 antibody
    • AMPK subunit gamma-3 antibody
    • AMPKG3 antibody
    • PRKAG3 antibody
    • Protein kinase AMP activated gamma 3 non catalytic subunit antibody
    see all

Anti-AMPK gamma 3 antibody 画像

  • Anti-AMPK gamma 3 antibody (ab38228) at 1/100 dilution + mouse brain tissue lysate

    developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38228 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-AMPK gamma 3 antibody (ab38228) 使用論文

ab38228 has not yet been referenced specifically in any publications.