製品の概要

  • 製品名Anti-AMPK gamma 1 antibody [Y308]
    AMPK gamma 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y308] to AMPK gamma 1
  • 特異性ab32508 recognises 5’-AMP-activated protein kinase (AMPK).
  • アプリケーション適用あり: WB, Flow Cyt, IPmore details
    適用なし: ICC or IHC
  • 種交差性
    交差種: Mouse, Rat, Human, African Green Monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • ポジティブ・コントロール
    • Jurkat cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration. PubMed: 23612997
  • 追加情報Is unsuitable for ICC or IHC.
  • ターゲット情報

    • 機能AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
    • 配列類似性Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
      Contains 4 CBS domains.
    • ドメインThe AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
      The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
    • 翻訳後修飾Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 5' AMP activated protein kinase gamma 1 subunit antibody
      • 5' AMP activated protein kinase subunit gamma 1 antibody
      • 5''-AMP-activated protein kinase subunit gamma-1 antibody
      • AAKG1_HUMAN antibody
      • AMP activated protein kinase noncatalytic gamma 1 subunit antibody
      • AMPK gamma 1 chain antibody
      • AMPK gamma1 antibody
      • AMPK subunit gamma-1 antibody
      • AMPKg antibody
      • MGC8666 antibody
      • PRKAG 1 antibody
      • PRKAG1 antibody
      • Protein kinase AMP activated gamma 1 non catalytic subunit antibody
      • protein kinase, AMP-activated, noncatalytic gamma-1 antibody
      see all

    Anti-AMPK gamma 1 antibody [Y308] 画像

    • All lanes : Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HEK293 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      Alexa Fluor® 690-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 38 kDa
      Observed band size : 38 kDa


      Exposure time : 5 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 38 kDa
      Observed band size : 38 kDa
    • AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Bands: 37kDa: AMPK gamma 1.

    Anti-AMPK gamma 1 antibody [Y308] (ab32508) 使用論文

    This product has been referenced in:
    • Lee KM  et al. Functional Effects of a Pathogenic Mutation in Cereblon (CRBN) on the Regulation of Protein Synthesis via the AMPK-mTOR Cascade. J Biol Chem 289:23343-52 (2014). WB ; Mouse . Read more (PubMed: 24993823) »
    • Mortensen B  et al. Effect of birth weight and 12 weeks of exercise training on exercise-induced AMPK signaling in human skeletal muscle. Am J Physiol Endocrinol Metab 304:E1379-90 (2013). IP ; Human . Read more (PubMed: 23612997) »

    See all 6 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample African Green Monkey Cell lysate - whole cell (COS7)
    Specification COS7
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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    投稿 Apr 03 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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    Abcam user community

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    投稿 Nov 25 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10% tris glycine)
    Sample Mouse Cell lysate - other (mouse primary hepatocytes)
    Specification mouse primary hepatocytes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
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    投稿 May 13 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - nuclear (AMPK gama 1 infected cells)
    Loading amount 20 µg
    Specification AMPK gama 1 infected cells
    Gel Running Conditions Reduced Denaturing (12% SDS PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Sep 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Loading amount 20 µg
    Specification Skeletal muscle
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Mr. Jonas Treebak

    Verified customer

    投稿 Oct 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"