Anti-AMPK gamma 1 抗体 [Y307] (ab32382)

製品の概要

  • 製品名Anti-AMPK gamma 1 antibody [Y307]
    AMPK gamma 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y307] to AMPK gamma 1
  • アプリケーション適用あり: WB, ICC, Flow Cyt, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues from the N terminus of human AMPK gamma 1 subunit.

  • エピトープab32382 reacts with an epitope located in the N terminal region of AMPK gamma 1.
  • ポジティブ・コントロール
    • Jurkat cell lysate and HeLa cell lysate, human skeletal muscles
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-AMPK gamma 1 antibody (Alexa Fluor® 488) [Y307] (ab205423)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
ICC 1/50 - 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
  • 配列類似性Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
    Contains 4 CBS domains.
  • ドメインThe AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
    The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
  • 翻訳後修飾Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5' AMP activated protein kinase gamma 1 subunit antibody
    • 5' AMP activated protein kinase subunit gamma 1 antibody
    • 5''-AMP-activated protein kinase subunit gamma-1 antibody
    • AAKG1_HUMAN antibody
    • AMP activated protein kinase noncatalytic gamma 1 subunit antibody
    • AMPK gamma 1 chain antibody
    • AMPK gamma1 antibody
    • AMPK subunit gamma-1 antibody
    • AMPKg antibody
    • MGC8666 antibody
    • PRKAG 1 antibody
    • PRKAG1 antibody
    • Protein kinase AMP activated gamma 1 non catalytic subunit antibody
    • protein kinase, AMP-activated, noncatalytic gamma-1 antibody
    see all

Anti-AMPK gamma 1 antibody [Y307] 画像

  • All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • This image shows paraffin embedded human skeletal muscle stained with 1/250 ab23282.
  • All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

    Lane 1 : HeLa whole cell lystate - Control Si
    Lane 2 : HeLa whole cell lystate - treated with AMPK gamma 1 siRNA A for 36 hours
    Lane 3 : HeLa whole cell lystate - treated with AMPK gamma 1 siRNA B for 36 hours

    Secondary
    HRP-conjugated Donkey polyclonal to rabbit IgG at 1/3000 dilution

    Predicted band size : 38 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-AMPK gamma 1 antibody [Y307] (ab32382) 使用論文

ab32382 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100000 cells
Specification HeLa
Treatment AMPK gamma1 siRNA for 36hrs
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Feb 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"