Anti-AMPK beta 1 (phospho S181) 抗体 [EP2181Y] (ab68206)

製品の概要

  • 製品名Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]
    AMPK beta 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2181Y] to AMPK beta 1 (phospho S181)
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta 1.

  • ポジティブ・コントロール
    • Human brain tissue lysate (cells untreated or treated with AP), HeLa cells and human skeletal muscle tissue
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/2000 - 1/20000. Detects a band of approximately 32, 45 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
    • 配列類似性Belongs to the 5'-AMP-activated protein kinase beta subunit family.
    • ドメインThe glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
    • 翻訳後修飾Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1300015D22Rik antibody
      • 5''-AMP-activated protein kinase subunit beta-1 antibody
      • AAKB1_HUMAN antibody
      • AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
      • AMP-activated, noncatalytic, beta-1 antibody
      • AMPK antibody
      • AMPK beta 1 chain antibody
      • AMPK subunit beta-1 antibody
      • AMPK-BETA-1 antibody
      • AMPKb antibody
      • AU021155 antibody
      • E430008F22 antibody
      • HAMPKb antibody
      • MGC17785 antibody
      • PRKAB1 antibody
      • Protein kinase AMP activated non catalytic subunit beta 1 antibody
      see all

    Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] 画像

    • All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/20000 dilution

      Lane 1 : human brain tissue lysate (cells were untreated)
      Lane 2 : human brain tissue lysate (cells were treated with AP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 32 and 45 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab68206 at 1/100-1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded human skeletal muscle using ab68206 at 1/100-1/250 dilution.

    Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) 使用論文

    ab68206 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"