Anti-AMPK beta 1 (phospho S181) 抗体 [EP2181Y] (ab68206)

製品の概要

  • 製品名
    Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]
    AMPK beta 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2181Y] to AMPK beta 1 (phospho S181)
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta 1.

  • ポジティブ・コントロール
    • Human brain tissue lysate (cells untreated or treated with AP), HeLa cells and human skeletal muscle tissue
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/2000 - 1/20000. Detects a band of approximately 32, 45 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
    • 配列類似性
      Belongs to the 5'-AMP-activated protein kinase beta subunit family.
    • ドメイン
      The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
    • 翻訳後修飾
      Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1300015D22Rik antibody
      • 5''-AMP-activated protein kinase subunit beta-1 antibody
      • 5'-AMP-activated protein kinase beta-1 subunit antibody
      • AAKB1_HUMAN antibody
      • AMP-activated protein kinase beta subunit antibody
      • AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
      • AMP-activated, noncatalytic, beta-1 antibody
      • AMPK antibody
      • AMPK beta 1 chain antibody
      • AMPK subunit beta-1 antibody
      • AMPK-BETA-1 antibody
      • AMPKb antibody
      • AU021155 antibody
      • E430008F22 antibody
      • HAMPKb antibody
      • MGC17785 antibody
      • PRKAB1 antibody
      • Protein kinase AMP activated non catalytic subunit beta 1 antibody
      • protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody
      • protein kinase, AMP-activated, noncatalytic, beta-1 antibody
      see all

    画像

    • All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/20000 dilution

      Lane 1 : human brain tissue lysate (cells were untreated)
      Lane 2 : human brain tissue lysate (cells were treated with AP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 32 and 45 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab68206 at 1/100-1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded human skeletal muscle using ab68206 at 1/100-1/250 dilution.

    参考文献

    ab68206 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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