The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 30 kDa).
Use at an assay dependent concentration.
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Belongs to the 5'-AMP-activated protein kinase beta subunit family.
The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
This image shows human skeletal muscle tissue stained with ab55311 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.
Western blot - AMPK beta 1 (phospho S181) antibody (ab55311)
All lanes : Anti-AMPK beta 1 (phospho S181) antibody (ab55311) at 1/500 dilution
Lane 1 : Jurkat cell extract Lane 2 : Jurkat cell extract, with the immunising phosphopeptide.