Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) 抗体 (ab72845)

製品の概要

  • 製品名Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody
  • 製品の詳細
    Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
  • 特異性Antibody detects endogenous AMPK alpha only when phosphorylated at SDGEFLR(pT)SCGSPNY. The antibody detects both alpha1 and alpha2 isoforms.
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Rat, Chicken, Human
    交差が予測される動物種: Mouse, Cow, Dog非交差種: Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human AMPK alpha 1 (phospho T183). Also within AMPK alpha 2 (phospho T172). It is identical among Human, mouse, rat, chicken, bovine, dog, and insect species.

  • ポジティブ・コントロール
    • Serum starved HEK293 whole cell lysate.

アプリケーション

Our Abpromise guarantee covers the use of ab72845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody 画像

  • All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845) at 1/500 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Whole cell lysate from serum starved HEK 293 cells
    Lane 3 : BSA negative control


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845) 使用論文

This product has been referenced in:
  • Zou D  et al. Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats. PLoS One 10:e0124727 (2015). WB ; Rat . Read more (PubMed: 25919288) »
  • Garvey SM  et al. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiol Rep 3:N/A (2015). WB ; Rat . Read more (PubMed: 25716928) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Treatment control shRNA and SNF1A shRNA embryos
Specification 0-4hr embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Richelle Sopko

Verified customer

投稿 Mar 04 2014

free of charge replacement sent.

Thank you for calling Abcam earlier today.
I am sorry about the issues you have been having with ab72845 in western blot. As we discussed I am sending you a new vial of the antibody and you should receive that tomorrow morning. For your reference, ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Gracias por contactarnos y tomaros la molestia de responder nuestro cuestionario.   He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría haceros para intentar mejorar los resultados del anticuerpo. De cualquier manera, al e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"