AMPK alpha 1 overexpression 293T lysate (whole cell) (ab94311)

製品の概要

  • 製品名AMPK alpha 1 overexpression 293T lysate (whole cell)
  • 製品の詳細
    AMPK alpha 1 overexpression lysate
  • 特記事項ab94311 is a 293T cell transfected lysate in which Human AMPK alpha 1 has been transiently over-expressed using a pCMV-AMPK alpha 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

アプリケーション

Our Abpromise guarantee covers the use of ab94311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

AMPK alpha 1 overexpression 293T lysate (whole cell) 画像

  • ab94311 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-AMPK alpha 1 antibody (ab89214) at 1/500 dilution

    Lane 1 : AMPK alpha 1 293T Transfected Lysate - Positive Control (ab94311)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

AMPK alpha 1 overexpression 293T lysate (whole cell) (ab94311) 使用論文

ab94311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"