Anti-AMPK alpha 1 + AMPK alpha 2 抗体 [34.2] (ab80039)
製品の概要
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製品名Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2]
AMPK alpha 1 + AMPK alpha 2 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2
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特異性Recognises both the alpha 1 and the alpha 2 AMPK
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アプリケーション適用あり: ELISA, WB, IP, IHC-Frmore details
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種交差性交差種: Mouse, Rat, Human, Drosophila melanogaster
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免疫原
29 amino acid peptide designed within the N-terminal catalytic domains of Drosophila AMPK alpha 1 and 2
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: rat heart tissue; human brain tissue; HepG2 whole cell; HEK293 whole cell.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Some batches contain 0.4M arginine. Please contact Scientific Support for further information. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノモノクローナル
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クローン名34.2
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アイソタイプIgG2a
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab80039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Abcam recommends using 3% milk as the blocking agent. |
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| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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機能Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
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配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. -
ドメインThe AIS (autoinhibitory sequence) region some sequence similarity with the ubiquitin-associated domains and represses kinase activity.
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翻訳後修飾Ubiquitinated.
Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. -
細胞内局在Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters.
- Information by UniProt
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参照データベース
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 600497 Human
- Omim: 602739 Human
see all -
別名
- 5''-AMP-activated protein kinase catalytic subunit alpha-1 antibody
- AAPK1_HUMAN antibody
- ACACA kinase antibody
see all
Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] 画像
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![Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)](http://a.abcam.com/ps/datasheet/images/80/ab80039/AMPK-alpha-1-AMPK-alpha-2-Primary-antibodies-ab80039-9.jpg)
Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 62 kDa
Observed band size : 62 kDa
Additional bands at : 75 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutesAbcam recommends using milk as the blocking agent when running western blots of ab80039. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab80039 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution. -
![Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 [34.2] antibody (ab80039)](http://a.abcam.com/ps/products/80/ab80039/Images/ab80039-228394-ab80039wb.jpg)
Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 [34.2] antibody (ab80039)This image is courtesy of an Abreview submitted by Brandon d'EonAnti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1/1000 dilution + Mouse 3T3-L1 adipocytes whole cell lysate at 25 µg
Secondary
HRP-conjugated goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 62 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutesThis image is courtesy of an Abreview submitted by Brandon d'Eon
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![Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 [34.2] antibody (ab80039)](http://a.abcam.com/ps/products/80/ab80039/Images/ab80039-213889-ab8003939832copy.png)
Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 [34.2] antibody (ab80039)Image courtesy of Richelle Sopko, Harvard University, U.S.A.All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1/1000 dilution
Lane 1 : Lysate of 0-4 hour Drosophila embryos expressing control shRNA.
Lane 2 : Lysate of 0-4 hour Drosophila embryos expressing SNF1A shRNA.
Secondary
Sheep anti-mouse IgG conjugated to HRP at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 62 kDa
Exposure time : 10 minutesImage courtesy of Richelle Sopko, Harvard University, U.S.A.
Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) 使用論文
This product has been referenced in:
- Omura J et al. Protective Roles of Endothelial AMP-Activated Protein Kinase Against Hypoxia-Induced Pulmonary Hypertension in Mice. Circ Res 119:197-209 (2016). Read more (PubMed: 27217398) »
- Jiao Y et al. The Fto Gene Regulates the Proliferation and Differentiation of Pre-Adipocytes in Vitro. Nutrients 8:102 (2016). WB ; Mouse . Read more (PubMed: 26907332) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"