Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)


  • 製品名
    Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
    Ammonia キット 製品一覧
  • サンプルの種類
    Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • アッセイタイプ
  • 検出感度
    < 10 µM
  • 全工程の試験時間
    0h 30m
  • 製品の概要

    Abcam's Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.


    Store the kit at +4°C.

  • アプリケーション
    適用あり: Functional Studiesmore details


  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Ammonia Reagent 1 Amber 1 x 8ml
    Ammonia Reagent 2 Clear 1 x 4ml
    Ammonium Chloride Standard Yellow 1 x 0.1ml
  • 研究分野
  • 関連性
    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.


Our Abpromise guarantee covers the use of ab102509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.


  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Ammonia measured in biological fluids showing quantity (mM) in tested samples. Samples were diluted 10-1000 fold.



ab102509 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.
In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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Thank you for contacting us. I have spoken with the lab regarding the need to deproteinize mouse urine samples with this kit. They would still strongly recommned this step to attain the best possible data with this product. I hope this informatio...

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Thank you for contacting us.

This product may be used to measure ammonium. In thisassay, the standards used are ammonium. Adjustment of the protocol is not required and detection levels will be the same.

I hope this infor...

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Thank you for your inquiry.

The most common reason for a low standard curve is a mistake when preparing the dilutions. I can tell from my on personal experience that a 10 times calculation mistake is especially easy to make.


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Thank you for calling Abcam earlier today

I have talked to the lab and the kit components are stable for at least 6 months (and up to 12 month) from the shipping date, if stored at the right temp and conditions.

Please let me know i...

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Thank you for contacting us. The kits you listed are appropriate for your samples, though we recommend ab83360 over ab102509 for assay of ammonia in urine samples. None have been published, to our knowledge. For a quote, we will need a shippin...

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Thank you for your inquiry. The lab confirmed that yes, these assay kits are compatible with serum samples from mammalian samples but they did not provide an exact protocol. I've included our general serum sample protocol below: For serum: Coll...

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