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Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of ammonia and ammonium. In the assay, ammonia and ammonium are converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of total ammonia and ammonium, which is much more sensitive than measuring ammonia with a NADPH based assay.
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Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.
|Ammonia Assay Buffer||WM||1 x 25ml|
|Converting Enzyme (Lyophilized)||Blue||1 vial|
|Enzyme Mix (lyophilized)||Green||1 vial|
|NH4Cl Standard||Yellow||1 x 100µl|
|OxiRed Probe in DMSO||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab83360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Ammonia measured in cell culture medium (range of dilution 1:10-1:200) and control medium (range of dilution 1:1-1:20), background signal subtracted (duplicates +/- SD).
Ammonia measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
Ammonia measured in cell lysates, background signal subtracted (duplicates +/- SD).
Ammonia measured in biological fluids (range of dilution 1:1-1:100), background signal subtracted (duplicates +/- SD).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"