Aminopotentidine Fluorescent ligand (Red) (ab118175)

製品の概要

  • 製品名Aminopotentidine Fluorescent ligand (Red)
  • 製品の詳細

    Fluorescent H2 antagonist

  • 生理活性の詳細Fluorescent H2 antagonist (pKD values are 8.94 and 7.30 at H2 and H3 receptors respectively). No intrinsic agonist activity in vitro. Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics.

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • 精製度> 97%

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab118175 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Small Molecule Fluorescent Optical Detection Use at an assay dependent concentration.
    Receptor Localization Use at an assay dependent concentration.
    Fluorescent Cell Imaging Use at an assay dependent concentration.

    Aminopotentidine Fluorescent ligand (Red) 画像

    • Left: ab118175 ligand (30 nM) binding to CHO cells expressing histamine H2 receptors. Right: Binding blocked by the unlabelled competitor ranitidine (10 µM). Nuclei have been counter-stained with Hoechst.
    • To determine the apparent KD for ab118175 at histamine H2 receptor, cells were treated with varying concentrations of histamine agonist alone, or in the presence of 1 µM ab118175, and the cyclic AMP-induced expression of SPAP measured.
      The apparent KD at H2 was calculated from the rightward shift of the agonist response curve in the presence of ab118175, compared to the response curve for the agonist alone. KD value for H2 was found to be 8.94 Antagonist -log KD.
    • To determine the apparent KD for ab118175 at histamine H3 receptor, cells were treated with varying concentrations of histamine agonist alone, or in the presence of 1 µM ab118175, and the cyclic AMP-induced expression of SPAP measured.
      The apparent KD at H3 was calculated from the rightward shift of the agonist response curve in the presence of ab118175, compared to the response curve for the agonist alone. The KD for ab118175 was found to be 7.3 Antagonist-log KD

    Aminopotentidine Fluorescent ligand (Red) (ab118175) 使用論文

    ab118175 has not yet been referenced specifically in any publications.

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