Anti-Aminoadipate aminotransferase 抗体 (ab89608)

製品の概要

  • 製品名
    Anti-Aminoadipate aminotransferase antibody
    Aminoadipate aminotransferase 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Aminoadipate aminotransferase
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Full length protein corresponding to amino acids 1-429 of Human Aminoadipate aminotransferase (AAH31068.1).

  • ポジティブ・コントロール
    • Human liver lysate, Aminoadipate aminotransferase transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.

ターゲット情報

  • 機能
    Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
  • 組織特異性
    Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
  • パスウェイ
    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
  • 配列類似性
    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2 aminoadipate aminotransferase antibody
    • 2 aminoadipate transaminase antibody
    • 2-aminoadipate aminotransferase antibody
    • 2-aminoadipate transaminase antibody
    • Aadat antibody
    • AADAT_HUMAN antibody
    • Aadt antibody
    • AI875679 antibody
    • Alpha aminoadipate aminotransferase antibody
    • Alpha-aminoadipate aminotransferase antibody
    • Aminoadipate aminotransferase antibody
    • EC 2.6.1.39 antibody
    • EC 2.6.1.7 antibody
    • KAT/AadAT antibody
    • KAT2 antibody
    • KATII antibody
    • Kynurenine oxoglutarate transaminase 2 antibody
    • Kynurenine aminotransferase II antibody
    • Kynurenine oxoglutarate aminotransferase II antibody
    • Kynurenine oxoglutarate transaminase II antibody
    • Kynurenine--oxoglutarate aminotransferase II antibody
    • Kynurenine--oxoglutarate transaminase II antibody
    • Kynurenine/alpha-aminoadipate aminotransferase mitochondrial [Precursor] antibody
    • Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial antibody
    • L kynurenine/alpha aminoadipate aminotransferase antibody
    see all

画像

  • Anti-Aminoadipate aminotransferase antibody (ab89608) at 1 µg/ml + Human liver lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Aminoadipate aminotransferase antibody (ab89608) at 1 µg/ml

    Lane 1 : Aminoadipate aminotransferase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab89608 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Rat Tissue lysate - whole (Hypothalamus)
Specification
Hypothalamus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (hippocampus)
Loading amount
20 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (9)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Jana Aradska

Verified customer

投稿 Aug 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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