Anti-Aminoadipate aminotransferase 抗体 (ab89608)

製品の概要

  • 製品名Anti-Aminoadipate aminotransferase antibody
    Aminoadipate aminotransferase 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Aminoadipate aminotransferase
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Full length protein corresponding to amino acids 1-429 of Human Aminoadipate aminotransferase (AAH31068.1).

  • ポジティブ・コントロール
    • Human liver lysate, Aminoadipate aminotransferase transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.

ターゲット情報

  • 機能Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
  • 組織特異性Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
  • パスウェイAmino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
  • 配列類似性Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2 aminoadipate aminotransferase antibody
    • 2 aminoadipate transaminase antibody
    • 2-aminoadipate aminotransferase antibody
    • 2-aminoadipate transaminase antibody
    • Aadat antibody
    • AADAT_HUMAN antibody
    • Aadt antibody
    • AI875679 antibody
    • Alpha aminoadipate aminotransferase antibody
    • Alpha-aminoadipate aminotransferase antibody
    • Aminoadipate aminotransferase antibody
    • EC 2.6.1.39 antibody
    • EC 2.6.1.7 antibody
    • KAT/AadAT antibody
    • KAT2 antibody
    • KATII antibody
    • Kynurenine oxoglutarate transaminase 2 antibody
    • Kynurenine aminotransferase II antibody
    • Kynurenine oxoglutarate aminotransferase II antibody
    • Kynurenine oxoglutarate transaminase II antibody
    • Kynurenine--oxoglutarate aminotransferase II antibody
    • Kynurenine--oxoglutarate transaminase II antibody
    • Kynurenine/alpha-aminoadipate aminotransferase mitochondrial [Precursor] antibody
    • Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial antibody
    • L kynurenine/alpha aminoadipate aminotransferase antibody
    see all

Anti-Aminoadipate aminotransferase antibody 画像

  • Anti-Aminoadipate aminotransferase antibody (ab89608) at 1 µg/ml + Human liver lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Aminoadipate aminotransferase antibody (ab89608) at 1 µg/ml

    Lane 1 : Aminoadipate aminotransferase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

Anti-Aminoadipate aminotransferase antibody (ab89608) 使用論文

ab89608 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Rat Tissue lysate - whole (Hypothalamus)
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (hippocampus)
Loading amount 20 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (9)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Jana Aradska

Verified customer

投稿 Aug 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"