Anti-AMIGO2 抗体 (ab84416)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AMIGO2
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A 15 amino acid peptide from near the C terminal residues of Human AMIGO2 (NM_181847)

  • ポジティブ・コントロール
    • Human breast carcinoma tissue.

法規制情報

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute in 200ul sterile H2O.
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 2% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab84416 is by a peptide affinity column.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500.
ICC/IF 1/100.

ターゲット情報

  • 機能
    Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
  • 組織特異性
    Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. AMIGO family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 細胞内局在
    Cell membrane. Nucleus. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adhesion molecule with Ig like domain 2 antibody
    • ALI 1 antibody
    • ALI1 antibody
    • Alivin 1 antibody
    • Alivin-1 antibody
    • Alivin1 antibody
    • AMGO2_HUMAN antibody
    • AMIGO 2 antibody
    • AMIGO-2 antibody
    • Amigo2 antibody
    • Amphoterin induced gene 2 antibody
    • Amphoterin induced protein 2 antibody
    • Amphoterin-induced gene and open reading frame 2 antibody
    • Amphoterin-induced protein 2 antibody
    • DEGA antibody
    • Differentially expressed in gastric adenocarcinoma antibody
    • Differentially expressed in gastric adenocarcinomas antibody
    • Transmembrane protein AMIGO 2 antibody
    • Transmembrane protein AMIGO2 antibody
    see all

画像

  • ab84416 at 1/100 dilution staining AMIGO2 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

参考文献

ab84416 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing (gradient 4-12% NuPAGE)
Loading amount
30 µg
Specification
melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 12 2016

Thank you for contacting us.
Your credit note ID is *****.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the...

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Thank you for your reply.

I would be happy to raise a discount code for testing ab84416 in Mouse samples with WB if you could please use Human fetal brain lysate or Human breast carcinoma tissue as positive control.

As two untested ...

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Thank you for your reply.

I am happy to hear you are interested in participating in our Abreview system. However, ab84416 has not been tested in WB and unfortunately the discount code is only applicable for only one untested species / applica...

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Thank you for your patience.

Both anti-AMIGO antibodies (ab84416 and ab84476) share the same immunogen sequence. In fact the difference between both antibodies is the purification process. While ab84476 is provided as whole antiserum, ab84416...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human umbilical vein endothelial cells)
Specification
Human umbilical vein endothelial cells
Fixative
Formaldehyde
Permeabilization
Yes - triton X-100
Username

Abcam user community

Verified customer

投稿 Oct 21 2011

DISCOUNT CODE: xxxxx Expiration date: 2012-01-06 I am very pleased to hear you would like to accept our offer and test ab84416 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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