製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AMH
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (SVDLRAERSV LIPETYQANN CQGVCGWPQS DRNPRYGNHV VLLLKMQARG) of Human AMH (NP_000470).

  • ポジティブ・コントロール
    • Human fetal small intestine lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • Anti muellerian hormone antibody
    • MIF antibody
    • MIS antibody
    • Muellerian inhibiting factor antibody
    • Mullerian inhibiting substance antibody
    see all

画像

  • Anti-AMH antibody (ab84952) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab84952 staining AMH in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

参考文献

This product has been referenced in:
  • Nistal M  et al. Sertoli cell dedifferentiation in human cryptorchidism and gender reassignment shows similarities between fetal environmental and adult medical treatment estrogen and antiandrogen exposure. Reprod Toxicol 42:172-9 (2013). Human . Read more (PubMed: 24012888) »

See 1 Publication for this product

レビューと Q&A

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Application
Western blot
Sample
Rat Cell lysate - whole cell (Testicular Sertoli cell)
Loading amount
10 µg
Specification
Testicular Sertoli cell
Treatment
100nm genistein and/or 100nm daidzein
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. India Napier

Verified customer

投稿 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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