製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AMH
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A 16 amino acid peptide from near the C terminal residues of Human AMH protein (NM_000479)

  • ポジティブ・コントロール
    • Human fetal testis lysates. IF: Lovo cell line

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF 1/200.

ターゲット情報

  • 関連性
    Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • Anti muellerian hormone antibody
    • MIF antibody
    • MIS antibody
    • Muellerian inhibiting factor antibody
    • Mullerian inhibiting substance antibody
    see all

画像

  • Anti-AMH antibody (ab84415) at 1/1000 dilution + human fetal testis lysates

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ICC/IF image of ab84415 stained Lovo cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84415, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab84415 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.

We generally recommend an expiry date of 10 years for antibodies stored at -80C. 50% Glycerol is recommended when abs stored at -80C.

We would not be able to guarantee the antibody after buffer exchange. T...

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Thank you for contacting us. The concentration of the current lot of this Protein A purified anti-AMH antibody is 10mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.  I have confirmed with the labs that made ab84415 and ab90241 that they have no information about anyone having ever tested these antibodies in paired applications.  I am sorry I could not provide any specific recommendatio...

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Thank you for your enquiry. ab84415 - The immunogen is a 15 amino acid peptide within the 50 amino acids of the C terminus of human AMH/MIS: LKMQVRGAALARPPCCVPTAYAGKLLISLSEERISAHHVPNMVATECGCR The exact sequence is considered to be proprieta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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