製品の概要

  • 製品名Anti-AMD1 antibody
    AMD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AMD1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GRMNSDCWYL YTLDFPESRV ISQPDQTLEI LMSELDPAVM DQFYMKDGVT) of human AMD1 (NP_001028231; Q5VXN4).

  • ポジティブ・コントロール
    • THP1 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. 38 kDa for isoform 1 and 21 kDa for isoform 2. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • パスウェイAmine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
  • 配列類似性Belongs to the eukaryotic AdoMetDC family.
  • 翻訳後修飾Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenosylmethionine decarboxylase 1 antibody
    • AdoMetDC antibody
    • AMD1 antibody
    • DCAM_HUMAN antibody
    • EC 4.1.1.50 antibody
    • FLJ26964 antibody
    • S adenosylmethionine decarboxylase 1 antibody
    • S adenosylmethionine decarboxylase proenzyme antibody
    • S-adenosylmethionine decarboxylase beta chain antibody
    • SAMDC antibody
    see all

Anti-AMD1 antibody 画像

  • Anti-AMD1 antibody (ab87661) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 46 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-AMD1 antibody (ab87661) 使用論文

ab87661 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab87661.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"