Anti-alpha Tubulin (phospho Y272) 抗体 [EP1334(2)Y] (ab76290)
製品の概要
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製品名Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
alpha Tubulin 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
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由来種Rabbit
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特異性This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
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アプリケーション適用あり: WB, ICCmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性交差種: Human
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免疫原
A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.
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ポジティブ･コントロール
- Jurkat cell lysates
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特記事項
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EP1334(2)Y
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アイソタイプIgG
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研究分野
関連製品
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab76290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/25000 - 1/50000. Predicted molecular weight: 54 kDa. | |
| ICC | 1/50 - 1/100. |
ターゲット情報
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機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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配列類似性Belongs to the tubulin family.
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翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在Cytoplasm > cytoskeleton.
- Information by UniProt
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参照データベース
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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別名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
画像
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![Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)](http://a.abcam.com/ps/products/76/ab76290/Images/ab76290.jpg)
Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution
Lane 1 : Jurkat cell lysates, untreated.
Lane 2 : Jurkat cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
参考文献
This product has been referenced in:
- Paech F et al. Hepatocellular Toxicity Associated with Tyrosine Kinase Inhibitors: Mitochondrial Damage and Inhibition of Glycolysis. Front Pharmacol 8:367 (2017). WB ; Human . Read more (PubMed: 28659801) »
- Yu Y et al. Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules. Cancer Cell 28:82-96 (2015). Read more (PubMed: 26096845) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"