Anti-alpha Tubulin (phospho Y272) 抗体 [EP1334(2)Y] (ab76290)

製品の概要

• 製品名
  Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
  alpha Tubulin 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
• 特異性
  This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
• アプリケーション
  適用あり: WB, ICCmore details
  適用なし: Flow Cyt,IHC-P or IP
• 種交差性
  交差種: Human
  
• 免疫原

  A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

• ポジティブ･コントロール
  • Jurkat cell lysates
• 特記事項

  A trial size is available to purchase for this antibody.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• バッファー
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 精製度
  Tissue culture supernatant
• ポリ/モノ
  モノクローナル
• クローン名
  EP1334(2)Y
• アイソタイプ
  IgG
• 研究分野
  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Microtubules

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • Tubulin

関連製品

• Positive Controls
  • Jurkat cytoplasmic extract lysate (ab14842)

アプリケーション

ターゲット情報

• 機能
  Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
• 配列類似性
  Belongs to the tubulin family.
• 翻訳後修飾
  Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
• 細胞内局在
  Cytoplasm > cytoskeleton.

• Target information above from: UniProt accession P68366 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 7277 Human
  • Omim: 191110 Human
  • SwissProt: P68366 Human
  • Unigene: 75318 Human
• 別名
  • Alpha-tubulin 1 antibody
  • ALS22 antibody
  • B ALPHA 1 antibody

  • bA408E5.3 antibody
  • H2 ALPHA antibody
  • Hum a tub1 antibody
  • Hum a tub2 antibody
  • LIS3 antibody
  • MGC171407 antibody
  • MGC55332 antibody
  • TBA4A_HUMAN antibody
  • Testis-specific alpha-tubulin antibody
  • TUBA1 antibody
  • TUBA1A antibody
  • tuba1l antibody
  • Tuba4a antibody
  • Tubulin alpha 1 chain antibody
  • Tubulin alpha antibody
  • Tubulin alpha-1 chain antibody
  • tubulin alpha-1B chain antibody
  • Tubulin alpha-4A chain antibody
  • Tubulin H2-alpha antibody
  • Tubulin, alpha 1 (testis specific) antibody
  • tubulin, alpha 1, like antibody
  • Tubulin, alpha 4a antibody
  • Tubulin, alpha, testis-specific antibody
  • Tubulin, alpha-1 antibody

  see all

プロトコール

• Western blot protocols
• Immunocytochemistry & immunofluorescence protocols