Anti-alpha Tubulin (phospho Y272) 抗体 [EP1334(2)Y] (ab76290)


  • 製品名Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
    alpha Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
  • 特異性This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

  • ポジティブ・コントロール
    • Jurkat cell lysates
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab76290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/25000 - 1/50000. Predicted molecular weight: 54 kDa.
ICC 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • 配列類似性Belongs to the tubulin family.
    • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • 細胞内局在Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha-tubulin 1 antibody
      • ALS22 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • MGC171407 antibody
      • MGC55332 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • tuba1l antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha-1 chain antibody
      • tubulin alpha-1B chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • tubulin, alpha 1, like antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] 画像

    • All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution

      Lane 1 : Jurkat cell lysates, untreated.
      Lane 2 : Jurkat cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) 使用論文

    This product has been referenced in:
    • Meshki J  et al. Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. PLoS One 6:e25332 (2011). WB . Read more (PubMed: 21966499) »

    See 1 Publication for this product

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