Anti-alpha Synuclein 抗体 [LB 509] (ab27766)

製品の概要

  • 製品名Anti-alpha Synuclein antibody [LB 509]
    alpha Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LB 509] to alpha Synuclein
  • 特異性This antibody recognizes human alpha synuclein. Clone LB 509 recognizes amino acids 115-122 of alpha synuclein and has been reported to be specific to human alpha synuclein (Jakes et al., Neurosci Lett., 1999). Therefore, we only guarantee this antibody to detect human alpha synuclein. However, several customer Abreviews have demonstrated positive staining in rodent samples.
  • アプリケーション適用あり: IHC-FoFr, ELISA, WB, IHC-Fr, IHC-P, Flow Cyt, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Lewy bodies purified from patients suffering dementia with Lewy bodies

  • エピトープAb27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha synuclein.
  • ポジティブ・コントロール
    • WB: full-length, recombinant alpha-synuclein protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/500.
ELISA Use at an assay dependent concentration.
WB 1/100 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-Fr 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Do not perform antigen retrieval.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
  • 組織特異性Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
  • 関連疾患Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
    Parkinson disease 1
    Parkinson disease 4
    Dementia Lewy body
  • 配列類似性Belongs to the synuclein family.
  • ドメインThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
  • 翻訳後修飾Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
    Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
    Ubiquitinated. The predominant conjugate is the diubiquitinated form.
    Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
  • 細胞内局在Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha synuclein antibody
    • Alpha-synuclein antibody
    • Alpha-synuclein, isoform NACP140 antibody
    • alphaSYN antibody
    • MGC105443 antibody
    • MGC110988 antibody
    • MGC127560 antibody
    • MGC64356 antibody
    • NACP antibody
    • Non A beta component of AD amyloid antibody
    • Non A4 component of amyloid antibody
    • Non A4 component of amyloid precursor antibody
    • Non-A beta component of AD amyloid antibody
    • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
    • Non-A4 component of amyloid precursor antibody
    • Non-A4 component of amyloid, precursor of antibody
    • OTTHUMP00000218549 antibody
    • OTTHUMP00000218551 antibody
    • OTTHUMP00000218552 antibody
    • OTTHUMP00000218553 antibody
    • OTTHUMP00000218554 antibody
    • PARK 1 antibody
    • PARK 4 antibody
    • PARK1 antibody
    • PARK4 antibody
    • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
    • Parkinson disease familial 1 antibody
    • PD 1 antibody
    • PD1 antibody
    • SNCA antibody
    • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
    • SYN antibody
    • Synuclein alpha antibody
    • Synuclein alpha 140 antibody
    • Synuclein, alpha (non A4 component of amyloid precursor) antibody
    • SYUA_HUMAN antibody
    see all

Anti-alpha Synuclein antibody [LB 509] 画像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa


    Exposure time : 8 minutes
  • IHC-P image of Alpha Synuclein (ab27766) staining in human brain samples with Multiple Systems Atrophy. The sections were subjected to heat-mediated antigen retrieval with citrate buffer. In addition, some slides received a 15 minute pre-treatment with Formic Acid. Sections were incubated in 20% serum for 30 minutes at +18°C to block non-specific protein-protein interactions. The sections were then incubated with ab27766 (1:400) for one hour at +18°C, followed by Biotin conjugated anti-mouse goat secondary antibody (1/200). Formic acid pre-treatment (15min) revealed more inclusions in MSA tissue.

    See Abreview

  • Overlay histogram showing PC12 cells stained with ab27766 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27766, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  • Anti-alpha Synuclein antibody [LB 509] (ab27766) at 1/10000 dilution (Sample: ab51189,0.1ug)

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa

Anti-alpha Synuclein antibody [LB 509] (ab27766) 使用論文

This product has been referenced in:
  • Chai YJ  et al. Munc18-1 is a molecular chaperone for a-synuclein, controlling its self-replicating aggregation. J Cell Biol 214:705-18 (2016). Read more (PubMed: 27597756) »
  • Flores-Cuadrado A  et al. a-Synuclein staging in the amygdala of a Parkinson's disease model: cell types involved. Eur J Neurosci 41:137-46 (2015). IHC . Read more (PubMed: 25345880) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (SH-SY5Y)
Specification SH-SY5Y
Treatment 50 ´M, 100 ´M or 200 ´M of dopamine with or without 100 nM of MG132 proteasome inhibitor for 72 h
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Dr. B Roberts

Verified customer

投稿 Feb 09 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Human Cell (SH-SY5Y)
Specification SH-SY5Y
Permeabilization Yes - 0.5% Tween
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 09 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (neuroblastoma)
Permeabilization No
Specification neuroblastoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 13 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing
Loading amount 100 µg
Specification brain tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Sep 18 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Permeabilization Yes - Methanol
Specification Brain sections
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Sep 16 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes)
Permeabilization Yes - Methanol
Specification Astrocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Neuroblastoma)
Loading amount 15 µg
Specification Neuroblastoma
Gel Running Conditions Reduced Denaturing
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50%
Username

Abcam user community

Verified customer

投稿 Apr 16 2013

Thank you for contacting us. The reviewwer used 90-100% formic acid for 10 minutes. Please let me kknow if you have further questions.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab98929 and one vial of ab77028with the order number 1150942.
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Thanks for sending the updated blot.

I believe its expected that Synuclein should be detected in the rat brain extracts, which it is not. There may be a band in your overexpressed Synuclein protein but it is difficult to determine with the la...

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