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Synthetic peptide MPVDPDNEAYEMPSEE of human synuclein (116-131) coupled to diphtheria toxoid with a cysteine residue attached to the N-terminal of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).
Our Abpromise guarantee covers the use of ab6162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use a concentration of 2 µg/ml.|
|Dot blot||Use a concentration of 0.5 µg/ml.|
|IHC-Fr||1/500 - 1/1000.|
|IHC-P||Use a concentration of 1 µg/ml. PubMed: 20106867|
|WB||Use a concentration of 0.5 µg/ml.|
IHC-FoFr image of Alpha Synuclein staining on alpha Synuclein Trasgenic mouse brain section stained using ab6162. The animal were perfuse with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton 0.3X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody ab 6162, 2 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 anti-sheep IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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