製品の概要

  • 製品名Anti-alpha Synuclein antibody
    alpha Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to alpha Synuclein
  • 特異性Human and rat alpha synuclein. Cross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not yet been tested.
  • アプリケーション適用あり: IHC-FoFr, Dot blot, IHC-Fr, IHC-P, WBmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide MPVDPDNEAYEMPSEE of human synuclein (116-131) coupled to diphtheria toxoid with a cysteine residue attached to the N-terminal of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use a concentration of 2 µg/ml.
Dot blot Use a concentration of 0.5 µg/ml.
IHC-Fr 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. PubMed: 20106867
WB Use a concentration of 0.5 µg/ml.
  • 追加情報Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • 組織特異性Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • 関連疾患Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • 配列類似性Belongs to the synuclein family.
    • ドメインThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • 翻訳後修飾Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • 細胞内局在Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • PD 1 antibody
      • PD1 antibody
      • SNCA antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    Anti-alpha Synuclein antibody 画像

    • ab6162 at a 1/40 dilution staining human brain tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with serum before incubation with ab6162 for 1 hour. A vector ABC kit was used to detect bound antibody.

      See Abreview

    • IHC-FoFr image of Alpha Synuclein staining on alpha Synuclein Trasgenic Mouse brain section stained using ab6162. The animal were perfuse with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton 0.3X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody ab 6162, 2 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 anti-sheep IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      See Abreview

    Anti-alpha Synuclein antibody (ab6162) 使用論文

    This product has been referenced in:
    • Surgucheva I  et al. New a- and ?-synuclein immunopathological lesions in human brain. Acta Neuropathol Commun 2:132 (2014). Read more (PubMed: 25209836) »
    • Kasahata N  et al. An 85-year old male with levodopa-responsive parkinsonism followed by dementia and supranuclear ophthalmoplegia caused by alzheimer-type pathology without Lewy bodies. J Alzheimers Dis 39:471-6 (2014). Read more (PubMed: 24217279) »

    See all 11 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (synaptosomes isolated from rat brain)
    Permeabilization Yes - 0.05 % Tween 20
    Specification synaptosomes isolated from rat brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Aug 24 2015

    Thank you for calling Abcam yesterday.

    I have heard back from the lab about the buffer of ab6162. All of our affinity purified antibody ultimately end up in PBS from which they are then lyophilized, thus all that is remaining are some buffer ...

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    Thank you very much for your enquiry.

    Unfortunately, we currently do not have a particular ready-to-useIHC Kit available that has been optimised for the use of a sheep polyclonal primary antibody with rat tissue.

    Therefore, if you d...

    Read More

    The production and formulation of this sheep antibody to alpha synuclein has not changed since 2009. I hope this is helpful. Please contact us again if you have any further questions.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Alpha Synuclein Transgenic Mouse brain)
    Specification Alpha Synuclein Transgenic Mouse brain
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% TritonX in 0.1% PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 May 18 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: Vector ABC
    Username

    Abcam user community

    Verified customer

    投稿 Jul 21 2006

    We assume you are staining for Lewy bodies in human diseased brains. We are using this antibody every week. The staining of Lewy bodies is really quite superb, either in paraffin or cryostat sections. The human tissue is fixed in Zamboni's fixativ...

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    This antibody works very well on paraffin sections. The antibody will need to be used at a concentration a little higher than for cryostat sections, about 1/500 to 1/1000.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"