Anti-alpha Synuclein 抗体 (ab21976)


  • 製品名Anti-alpha Synuclein antibody
    alpha Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to alpha Synuclein
  • 特異性The antibody to human alpha synuclein (aa 108-120) is specific and does not cross react with either beta or gamma synuclein.
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 108-120 of Human alpha Synuclein

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa
  • 特記事項

    This antibody achieves excellent staining of Lewy bodies in Parkinson’s disease and Dementia with Lewy bodies disease tissue and gives excellent staining of glial inclusions in Multi System Atrophy tissue.



Our Abpromise guarantee covers the use of ab21976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
IHC-P 1/1000 - 1/4000. Antigen retrieval has been used in characterisation but may not be necessary. 1) Slide holder containing 1mM EDTA at pH 8 is boiled in a microwave (~2min). 2) Slides are placed in holder and boiled for a further 10 min with the microwave on low. 3) Slides allowed to cool before use.
WB 1/2000 - 1/8000.


  • 機能May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
  • 組織特異性Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
  • 関連疾患Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
    Parkinson disease 1
    Parkinson disease 4
    Dementia Lewy body
  • 配列類似性Belongs to the synuclein family.
  • ドメインThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
  • 翻訳後修飾Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
    Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
    Ubiquitinated. The predominant conjugate is the diubiquitinated form.
    Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
  • 細胞内局在Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha synuclein antibody
    • Alpha-synuclein antibody
    • Alpha-synuclein, isoform NACP140 antibody
    • alphaSYN antibody
    • MGC105443 antibody
    • MGC110988 antibody
    • MGC127560 antibody
    • MGC64356 antibody
    • NACP antibody
    • Non A beta component of AD amyloid antibody
    • Non A4 component of amyloid antibody
    • Non A4 component of amyloid precursor antibody
    • Non-A beta component of AD amyloid antibody
    • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
    • Non-A4 component of amyloid precursor antibody
    • Non-A4 component of amyloid, precursor of antibody
    • OTTHUMP00000218549 antibody
    • OTTHUMP00000218551 antibody
    • OTTHUMP00000218552 antibody
    • OTTHUMP00000218553 antibody
    • OTTHUMP00000218554 antibody
    • PARK 1 antibody
    • PARK 4 antibody
    • PARK1 antibody
    • PARK4 antibody
    • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
    • Parkinson disease familial 1 antibody
    • PD 1 antibody
    • PD1 antibody
    • SNCA antibody
    • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
    • SYN antibody
    • Synuclein alpha antibody
    • Synuclein alpha 140 antibody
    • Synuclein, alpha (non A4 component of amyloid precursor) antibody
    • SYUA_HUMAN antibody
    see all

Anti-alpha Synuclein antibody 画像

  • ICC/IF image of ab21976 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab21976 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- Sheep (ab150177) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab21976 staining Lewy body in Dementia with Lewy body disease tissue at 1/1000 dilution by Immunohistochemistry (FFPE-sections). Secondary antibody biotinylated Donkey and sheep followed by Vector ABC and DAB substrate.

Anti-alpha Synuclein antibody (ab21976) 使用論文

ab21976 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (iPS cell derived neurons)
Permeabilization Yes - see blocking buffer
Specification iPS cell derived neurons
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde

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投稿 Jan 25 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10% SDS gel)
Sample Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))
Specification human iPS cell derived neurons (healthy/diseased)
Treatment quinolinic acid 72h (and non treated) 500nM
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

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投稿 Jun 19 2014

Thank you for your inquiry. This antibody has been characterized using antigen retreival in the following way. 1) Slide holder containing 1mM EDTA at pH 8 is boiled in a microwave (~2min). 2) Slides are placed in holder and boiled for a further 10...

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Thank you for your enquiry and for bringing this error to our attention. I have corrected the online datasheet, changed the immunogen information to state that the immunogen is amino acids 108-120 of alpha synuclein, and changed the specificity to s...

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