製品の概要

  • 製品名Anti-alpha smooth muscle Actin antibody
    alpha smooth muscle Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to alpha smooth muscle Actin
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (GST-tag) corresponding to Human alpha smooth muscle Actin aa 1-377.
    Database link: P62736

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • 配列類似性Belongs to the actin family.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody 画像

  • Anti-alpha smooth muscle Actin antibody (ab54723) at 1 µg/ml + Whole tissue lysate prepared from human omentum, serous carcinoma.

    Predicted band size : 42 kDa
  • Detection limit for GST tagged alpha smooth muscle actin is approximately 1 ng/ml as a capture antibody.

  • ab54723 at 1 - 10 ug/ml staining Alpha smooth muscle actin in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab54723 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat ant-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-alpha smooth muscle Actin antibody (ab54723) 使用論文

This product has been referenced in:
  • Feng Y  et al. Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer. Cell Death Dis 5:e1567 (2014). WB ; Human . Read more (PubMed: 25501828) »
  • Presser LD  et al. Activation of TGF-ß1 promoter by hepatitis C virus-induced AP-1 and Sp1: role of TGF-ß1 in hepatic stellate cell activation and invasion. PLoS One 8:e56367 (2013). WB ; Human . Read more (PubMed: 23437118) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela, HEK293)
Specification Hela, HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Feb 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"