製品の概要

  • 製品名
    Anti-alpha smooth muscle Actin antibody
    alpha smooth muscle Actin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to alpha smooth muscle Actin
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide corresponding to Human alpha smooth muscle Actin aa 3-12 (N terminal).
    Sequence:

    EEEDSTALVC


    Database link: P62736

  • ポジティブ・コントロール
    • Lysate prepared from human duodenum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

IHC-P Use a concentration of 2 - 4 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 20947685

Permeabilisation with ~0.2% Triton (in PBS) is recommended

ターゲット情報

  • 機能
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患
    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • 配列類似性
    Belongs to the actin family.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

画像

  • ab21027 staining alpha smooth muscle Actin in mouse pancreas by Immunohistochemistry  (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 3  hours at RT; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/100) for 16 hours. An Alexa Fluor® 647 donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody..

    See Abreview

  • All lanes : Anti-alpha smooth muscle Actin antibody (ab21027) at 0.1 µg/ml

    Lane 1 : Human Duodenum tissue lysates
    Lane 2 : Heart tissue lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 42 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • ab21027 staining alpha smooth muscle Actin in mouse femoral artery by Immunohistochemistry (PFA perfusion fixed frozen sections.) Tissue was fixed in formaldehyde, permeabilized using 0.1% Triton, blocked using 10% serum for 1 hour at 22°C and then incubated with ab21027 at a 1/300 dilution for 16 hours at 4 °C. The secondary used was an Alexa Fluor®488 rabbit anti-goat used at a 1/1000 dilution.

    See Abreview

  • Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + Human skeletal muscle lysate at 35 µg

    Predicted band size : 42 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + human duodenum lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human (top) and mouse (bottom) lung tissue sections labelling alpha smooth muscle Actin with ab21027 at 2.5µg/ml. Image shows staining of the alveolar walls. Trypsin-mediated antigen retrieval, HRP-staining. Bar in right lower corner represents 50um.

参考文献

This product has been referenced in:

See all 21 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Epithelial (hTert-RPE1))
Permeabilization
No
Specification
Epithelial (hTert-RPE1)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Methanol
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投稿 May 23 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, smooth muscle cell)
Permeabilization
Yes - sodium citrate buffer
Specification
lung, smooth muscle cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Dec 14 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (femoral artery)
Permeabilization
Yes - 0.1% Triton-X
Specification
femoral artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
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投稿 Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MSC)
Permeabilization
Yes - Triton 1%
Specification
MSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
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投稿 Jul 08 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - Triton 1%
Fixative
Paraformaldehyde
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投稿 Dec 02 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
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投稿 Nov 20 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Sample
Human Tissue sections (Mammary fat pad)
Specification
Mammary fat pad
Permeabilization
No
Fixative
Paraformaldehyde
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投稿 Apr 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Sample
Mouse Tissue sections (Mammary fat pad)
Specification
Mammary fat pad
Permeabilization
No
Fixative
Paraformaldehyde
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投稿 Apr 08 2014

Application
IHC - Wholemount
Sample
Mouse Tissue (Mammary fat pad)
Specification
Mammary fat pad
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投稿 Apr 08 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - Triton-X
Fixative
Acetone
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投稿 Apr 07 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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