Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Calvarial bone)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Calvarial bone
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS pH 7.4
Concentration
10 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain Elite ABC universal Kit
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Sections were incubated with peroxide, horse serum (10%), BSA (5%), and avidin/biotin blocking steps before incubation of primary antibody. Hematoxylin counter staining was used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jakob Townsend
Verified customer
投稿 Jan 05 2016