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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Overview
Product name
Anti-alpha smooth muscle Actin antibody
alpha smooth muscle Actin 抗体 (20件) 一覧
Description
Rabbit polyclonal to alpha smooth muscle Actin
Specificity
This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. The other actins, such as beta- and gamma-cytoplasmic, striated muscle, and myocardium are not stained by this antibody.
Tested applications
IHC-FoFr, ICC, ICC/IF, WB, ELISA, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Guinea pig, Cow, Human, Pig
Immunogen
Synthetic peptide (Human) corresponding to N-terminus of smooth muscle alpha actin.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Smooth Muscle Cells
Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
(enlarge)
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Western blot - alpha smooth muscle Actin antibody (ab5694)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab5694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution.
ICC: Use at an assay dependent concentration.
ICC/IF: 1/100
WB: Use a concentration of 0.5 - 2.0 µg/ml.Predicted molecular weight: 42 kDa.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
IHC-P: 1/100 - 1/400.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/200(PubMed: 18559614Fix with acetone.)
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similarities
Belongs to the actin family.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP62736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAT6 antibody
- ACTA_HUMAN antibody
- ACTA2 antibody
see all
Database links
- Entrez Gene: 423787 Chicken
- Entrez Gene: 515610 Cow
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P08023 Chicken
- SwissProt: P62736 Human
see all
Anti-alpha smooth muscle Actin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
Human Leiomyoma stained with ab5694.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
This picture shows formalin-fixed, paraffin embedded mouse intestine and mesentery, the optimal dilution is 1:1600 to 1:3200, incubation overnight at 4oC, counterstained with Hematoxylin.
This image was kindly supplied as part of the review by JQ Zhang.
Western blot - alpha smooth muscle Actin antibody (ab5694)
All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 30 kDa,35 kDa,37 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Please note that ab5694 does not appear to be specific to smooth muscle.
Immunohistochemistry (Frozen sections) - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)
Ab5694 positively staining smooth muscle cells in blood vessels and myoepithelial cells in the frozen tissue of cancerous human mammary gland (pink) at 1/100 dilution.
Secondary: CY5 conjugated goat anti rabbit (1/100).
Co immunostaining of glandular cell cytokeratin can be seen stained by FITC (green). Auto fluorescent erythrocytes that are present within blood vessels are shown (red), whilst the DAPI counter stain may clearly be seen staining nuclei (blue).
This image is courtesy of an Abreview submitted by on 22 August 2005. We do not have any further information relating to this image.
Western blot - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)
All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1/500 dilution
Lane 1 : Rat2 myofibroblasts (untreated before treatment-0 days)
Lane 2 : Rat2 myofibroblasts (untreated for 5 days)
Lane 3 : Rat2 myofibroblasts (treated with 1ng/mL TGF beta)
Lane 4 : Rat2 myofibroblasts (treated with 10ng/mL TGF beta)
Lane 5 : Positive control (NIH3T3)
Lane 6 : Negative control (MDA-MB-469 breast carcinoma cells)
Lysates/proteins at 10 µg per lane.
Secondary
Donkey anti rabbit (HRP) at 1/2500 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
This image is and edited version of an image submitted courtesy of an Abreview on 20 September 2005. We do not have any further information relating to this image.
Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)
ab5694 at 1/500 staining rat myofibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. A FITC conjugated goat anti-rabbit IgG was used as the secondary. Nuclei were counterstained with propidium iodide.
This image is courtesy of an Abreview submitted by Dr Jianyuan Chai
Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)
ab5694 staining human fetal heart cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - alpha smooth muscle Actin antibody (ab5694)
All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1/1000 dilution
Lane 1 : Lystates prepared from pig hear tissue from normal control animals
Lane 2 : Lystates prepared from pig hear tissue from normal control animals
Lane 3 : Lystates prepared from pig hear tissue from experimental animals
Lane 4 : Lystates prepared from pig hear tissue from experimental animals
Lysates/proteins at 4 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is a courtesy of Mario Torrado
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha smooth muscle Actin antibody (ab5694)
ab5694 staining alpha smooth muscle Actin in human skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocked with 10% serum for 1 hour at RT. The primary antibody was diluted 1/300 and incubated with sample in 2% serum for 15 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/500 as secondary antibody.
This image is courtesy of an Anonymous Abreview
References for Anti-alpha smooth muscle Actin antibody (ab5694)
This product has been referenced in:
- Moles Aet al. Cathepsin B overexpression due to Acid sphingomyelinase ablation promotes liver fibrosis in niemann-pick disease. J Biol Chem 287:1178-88 (2012).Read more (PubMed: 22102288) »
- Kanellakis Pet al. High-mobility group box protein 1 neutralization reduces development of diet-induced atherosclerosis in apolipoprotein e-deficient mice. Arterioscler Thromb Vasc Biol 31:313-9 (2011). IHC-Fr; Mouse.Read more (PubMed: 21088249) »
See all 49 publications for this product
Publishing research using ab5694? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"