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- alpha smooth muscle Actin antibody (ab5694)
- alpha smooth muscle Actin antibody (ab5694)
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- alpha smooth muscle Actin antibody (ab5694)
- alpha smooth muscle Actin antibody (ab5694)
(enlarge)


Western blot - alpha smooth muscle Actin antibody (ab5694)
Western blot - alpha smooth muscle Actin antibody (ab5694)
(enlarge)


Product Name 

alpha smooth muscle Actin antibody

 

alpha smooth muscle Actin 抗体 (17件) 一覧

Product type 

Primary antibodies

Description 

Rabbit polyclonal to alpha smooth muscle Actin

Immunogen 

Synthetic peptide (Human) corresponding to N-terminus of smooth muscle alpha actin.

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow, Pig

Specificity 

This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. The other actins, such as beta- and gamma-cytoplasmic, striated muscle, and myocardium are not stained by this antibody.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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• ELISA

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• ICC

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• ICC/IF

7 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IF

1 Abreview

- (no rating data)

• IHC-Fr

3 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IHC-P

17 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• WB

8 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


ICC/IF
(read Abreview)


ICC/IF
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ICC/IF
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ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
ICC/IF: 1/100.
IHC-P: 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/200 (Fix with acetone, PMID 18559614).
IHC-FoFr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 42 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin

Relevance 

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.

 

alpha smooth muscle Actin antibody (ab5694)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

515610    (Cow)
59    (Human)
11475    (Mouse)
81633    (Rat)

Omim

    

102620    (Human)

SwissProt

    

P08023    (Chicken)
P62739    (Cow)
P62736    (Human)
P62737    (Mouse)
P62738    (Rat)

Unigene

    

500483    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

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See below for alpha smooth muscle Actin antibody images, references, products related to ab5694 and other tools.

 

alpha smooth muscle Actin antibody images:

Product image  - alpha smooth muscle Actin antibody (ab5694)

- alpha smooth muscle Actin antibody (ab5694)

Human Leiomyoma stained with ab5694.

 

Product image  - alpha smooth muscle Actin antibody (ab5694)

- alpha smooth muscle Actin antibody (ab5694)

This picture shows formalin-fixed, paraffin embedded mouse intestine and mesentery, the optimal dilution is 1:1600 to 1:3200, incubation overnight at 4oC, counterstained with Hematoxylin.

This image was kindly supplied as part of the review by JQ Zhang.

 

Product image  Western blot - alpha smooth muscle Actin antibody (ab5694)

Western blot - alpha smooth muscle Actin antibody (ab5694)

All lanes : alpha smooth muscle Actin antibody (ab5694) at 1 µg/ml

Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 30 kDa,35 kDa,37 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Please note that ab5694 does not appear to be specific to smooth muscle.

 

Product image  Immunohistochemistry (Frozen sections) - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)

Immunohistochemistry (Frozen sections) - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)

Ab5694 positively staining smooth muscle cells in blood vessels and myoepithelial cells in the frozen tissue of cancerous human mammary gland (pink) at 1/100 dilution. Secondary: CY5 conjugated goat anti rabbit (1/100). Co immunostaining of glandular cell cytokeratin can be seen stained by FITC (green). Auto fluorescent erythrocytes that are present within blood vessels are shown (red), whilst the DAPI counter stain may clearly be seen staining nuclei (blue).

This image is courtesy of an Abreview submitted by on 22 August 2005. We do not have any further information relating to this image.

See Abreview

 

Product image  Western blot - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)

Western blot - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)

All lanes : alpha smooth muscle Actin antibody (ab5694) at 1/500 dilution

Lane 1 : Rat2 myofibroblasts (untreated before treatment-0 days)
Lane 2 : Rat2 myofibroblasts (untreated for 5 days)
Lane 3 : Rat2 myofibroblasts (treated with 1ng/mL TGF beta)
Lane 4 : Rat2 myofibroblasts (treated with 10ng/mL TGF beta)
Lane 5 : Positive control (NIH3T3)
Lane 6 : Negative control (MDA-MB-469 breast carcinoma cells)

Lysates/proteins at 10 µg per lane.

Secondary
Donkey anti rabbit (HRP) at 1/2500 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa


This image is and edited version of an image submitted courtesy of an Abreview on 20 September 2005. We do not have any further information relating to this image.

See Abreview

 

Product image  Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)

Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)

ab5694 at 1/500 staining rat myofibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. A FITC conjugated goat anti-rabbit IgG was used as the secondary. Nuclei were counterstained with propidium iodide.

This image is courtesy of an Abreview submitted by Dr Jianyuan Chai

See Abreview

 

Product image  Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)

Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)

ab5694 staining human fetal heart cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A  Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

 

Product image  Western blot - alpha smooth muscle Actin antibody (ab5694)

Western blot - alpha smooth muscle Actin antibody (ab5694)

All lanes : alpha smooth muscle Actin antibody (ab5694) at 1/1000 dilution

Lane 1 : Lystates prepared from pig hear tissue from normal control animals
Lane 2 : Lystates prepared from pig hear tissue from normal control animals
Lane 3 : Lystates prepared from pig hear tissue from experimental animals
Lane 4 : Lystates prepared from pig hear tissue from experimental animals

Lysates/proteins at 4 µg per lane.

Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

This image is a courtesy of Mario Torrado

See Abreview

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha smooth muscle Actin antibody (ab5694)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha smooth muscle Actin antibody (ab5694)

ab5694 staining alpha smooth muscle Actin in human skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocked with 10% serum for 1 hour at RT. The primary antibody was diluted 1/300 and incubated with sample in 2% serum for 15 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/500 as secondary antibody.

This image is courtesy of an Anonymous Abreview

See Abreview

 

References for alpha smooth muscle Actin antibody (ab5694)

Specific publication references   This product has been used in: (two most recent references)

Owens AP et al. Angiotensin II induces a region-specific hyperplasia of the ascending aorta through regulation of inhibitor of differentiation 3. Circ Res 106:611-9 (2010). IHC-FoFr; Mouse. PubMed: 20019328

Madsen K et al. Angiotensin II promotes development of the renal microcirculation through AT1 receptors. J Am Soc Nephrol 21:448-59 (2010). WB; Rat. PubMed: 20056745


See all 44 publication references for this product.


If you publish research using ab5694 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to alpha smooth muscle Actin

 

alpha smooth muscle Actin antibody - more information

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alpha smooth muscle Actin antibody - related products:

Products for Immunocytochemistry

Subcellular Markers

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Positive Controls for alpha smooth muscle Actin antibody

ab7899

  

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

ab7909

  

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

 

Compatible Secondaries for alpha smooth muscle Actin antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal Secondary Antibody to Rabbit IgG - H&L

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

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alpha smooth muscle Actin antibody - other tools:

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