Application
Western blot
Sample
Mouse Tissue lysate - whole (fibrotic mouse liver)
Loading amount
20 µg
Specification
fibrotic mouse liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.03% TBST with 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Additional data
Additional Notes
Weak bands compared to GADPH (lower bands~40kD).
Abcam response
Thank you for your review. We would expect the GAPDH band to be much stronger than the alpha smooth muscle actin, as it is much more highly expressed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 15 2012