Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Heart)
Specification
Heart
Fixative
Acetone
Permeabilization
Yes - 0.3 % Triton in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey pAb Goat FITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/1000
Additional data
Additional Notes
The green color indicates the staining of alpha smooth muscle actin. The blue color indicates staining of nuclei using DAPI.
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投稿 Dec 15 2011