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Synthetic peptide derived from N-terminus of Human skeletal muscle Actin. This sequence is identical in Human, Rat, Mouse, Dog, Bovine, Guinea pig, Sheep and Frog origins.
Our Abpromise guarantee covers the use of ab113417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||Use a concentration of 2 - 5 µg/ml. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|