Anti-alpha Internexin 抗体 [EP676Y] (ab40758)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP676Y] to alpha Internexin
- Suitable for: WB, IP, IHC-FoFr, Flow Cyt, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha Internexin antibody [EP676Y]
alpha Internexin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP676Y] to alpha Internexin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-FoFr, Flow Cyt, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
非交差種: Apteronotus leptorhynchus -
免疫原
Synthetic peptide within Human alpha Internexin aa 400-500. The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa, SHSY5Y cell lysate, human brain lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP676Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40758の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/5000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 66 kDa).
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IP |
Use at an assay dependent concentration.
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/250 - 1/500.
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特記事項 |
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WB
1/5000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 66 kDa). |
IP
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
ターゲット情報
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機能
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. -
組織特異性
Found predominantly in adult CNS. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation. -
翻訳後修飾
O-glycosylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 9118 Human
- Entrez Gene: 226180 Mouse
- Entrez Gene: 24503 Rat
- Omim: 605338 Human
- SwissProt: Q16352 Human
- SwissProt: P46660 Mouse
- SwissProt: P23565 Rat
- Unigene: 500916 Human
see all -
別名
- 66 kDa neurofilament protein antibody
- AINX_HUMAN antibody
- Alpha Inx antibody
see all
画像
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All lanes : Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : INA knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaLanes 1-4: Merged signal (red and green). Green - ab40758 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.
ab40758 Anti-alpha Internexin antibody [EP676Y] was shown to specifically react with alpha Internexin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265264 (knockout cell lysate ab257482) was used. Wild-type and alpha Internexin knockout samples were subjected to SDS-PAGE. ab40758 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg
Predicted band size: 66 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab40758 は 8 報の論文で使用されています。
- Morandell J et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nat Commun 12:3058 (2021). PubMed: 34031387
- Li S et al. The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein. Sci Rep 11:14001 (2021). PubMed: 34234233
- Iqbal J et al. Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention. J Neurosci Res 96:1798-1815 (2018). PubMed: 30054946
- Armao D et al. Autonomic nervous system involvement in the giant axonal neuropathy (GAN) KO mouse: implications for human disease. Clin Auton Res 26:307-13 (2016). PubMed: 27369358
- Ayyadevara S et al. Proteins that mediate protein aggregation and cytotoxicity distinguish Alzheimer's hippocampus from normal controls. Aging Cell 15:924-39 (2016). IHC-P ; Human . PubMed: 27448508
- Xing R et al. Spatial memory impairment by TRPC1 depletion is ameliorated by environmental enrichment. Oncotarget 7:27855-73 (2016). WB . PubMed: 27034165
- Coffey RT et al. Ubiquilin-mediated Small Molecule Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling. J Biol Chem 291:5221-33 (2016). PubMed: 26740621
- Louis ED et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett 518:49-54 (2012). PubMed: 22561033