リコンビナント
RabMAb

Anti-alpha Internexin 抗体 [EP676Y] (ab40758)

製品の概要

  • 製品名
    Anti-alpha Internexin antibody [EP676Y]
    alpha Internexin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP676Y] to alpha Internexin
  • アプリケーション
    適用あり: IHC-FoFr, WB, Flow Cyt, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    非交差種: Apteronotus leptorhynchus
  • 免疫原

    Synthetic peptide corresponding to residues in the tail domain (Human)

  • ポジティブ・コントロール
    • SHSY5Y cell lysate, human brain
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP676Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 66 kDa).
Flow Cyt 1/25.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 機能
    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • 組織特異性
    Found predominantly in adult CNS.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • 翻訳後修飾
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

画像

  • Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg

    Predicted band size : 66 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab40758 staining alpha Internexin in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with 0.01% Triton X-100, blocked with 5% serum for 2 hours at 4°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/250) at 4°C for 10 hours. An undiluted Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.
  • ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.

参考文献

This product has been referenced in:
  • Ayyadevara S  et al. Proteins that mediate protein aggregation and cytotoxicity distinguish Alzheimer's hippocampus from normal controls. Aging Cell 15:924-39 (2016). IHC-P ; Human . Read more (PubMed: 27448508) »
  • Xing R  et al. Spatial memory impairment by TRPC1 depletion is ameliorated by environmental enrichment. Oncotarget 7:27855-73 (2016). WB . Read more (PubMed: 27034165) »

See all 4 Publications for this product

レビューと Q&A

Application
IHC - Wholemount
Sample
Human Tissue (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

投稿 Dec 29 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Mixed Glial cells)
Specification
Mixed Glial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.01% Triton X100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 17 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Rat Cell (Glial Cells)
Specification
Glial Cells
Permeabilization
Yes - Saponin
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 13 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Feb 06 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2012


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Vlady Pavlova

Verified customer

投稿 Dec 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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