製品の概要

  • 製品名
    Anti-alpha Internexin antibody
    alpha Internexin 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to alpha Internexin
  • 由来種
    Chicken
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Cat, Human
  • 免疫原

    Recombinant full length protein expressed in E. coli.

  • ポジティブ・コントロール
    • ICC/IF: Rat cerebellum. WB: Rat brain and spinal cord; Mouse brain and spinal cord; Cow spinal cord; Pig spinal cord.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 10mM Sodium Azide.
  • Concentration information loading...
  • 特記事項(精製)
    This antibody was purified from egg yolk by chloroform delipidation followed by PEG precipitation.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 66-68 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/1000.

ターゲット情報

  • 機能
    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • 組織特異性
    Found predominantly in adult CNS.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • 翻訳後修飾
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

画像

  • Immunofluorescent analysis of a rat cerebellum section stained with ab24523 (Red) at 1/5000 dilution in immunohistochemical analysis. counterstained with antibody against myelin basic protein (Green). Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab24523 stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.

  • All lanes : Anti-alpha Internexin antibody (ab24523) at 1/10000 dilution

    Lane 1 : Protein standard (red)
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse spinal cord tissue lysate
    Lane 6 : Cow spinal cord tissue lysate
    Lane 7 : Pig spinal cord tissue lysate

    Predicted band size: 55 kDa



    ab24523 reveals the α-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.

参考文献

ab24523 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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