Anti-alpha Internexin 抗体 [2E3] (ab7654)

製品の概要

  • 製品名Anti-alpha Internexin antibody [2E3]
    alpha Internexin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E3] to alpha Internexin
  • 特異性specifically recognizes a-internexin.
  • アプリケーション適用あり: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: all Mammals
  • 免疫原

    Recombinant full length protein (Rat).

  • エピトープC-terminal non-helical extension of the protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 66 kDa.
Flow Cyt 1/100.
ICC/IF 1/500.
IHC-P 1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • 組織特異性Found predominantly in adult CNS.
  • 配列類似性Belongs to the intermediate filament family.
  • 発生段階Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • 翻訳後修飾O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

Anti-alpha Internexin antibody [2E3] 画像

  • Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
  • All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)

    Lane 1 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lane 2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lane 3 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lane 4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lane 5 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lane 6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lane 7 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lane 8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lane 9 : Crude homogenate of rat facial nucleus at 28 days after experimental injury
    Lane 10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury


    Predicted band size : 66 kDa
    Observed band size : 66 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab7654 staining alpha Internexin in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA, pH 9.0 (100°C for 20 minutes). Samples were incubated with primary antibody (1/2500) for 20 minutes at 25°C.

    See Abreview

Anti-alpha Internexin antibody [2E3] (ab7654) 使用論文

This product has been referenced in:
  • Rauch U  et al. Expression of intermediate filament proteins and neuronal markers in the human fetal gut. J Histochem Cytochem 54:39-46 (2006). Read more (PubMed: 16087706) »
  • Uchikado H  et al. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005). Read more (PubMed: 15883343) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 100C, 20 min
Sample Human Tissue sections (cerebral cortex)
Specification cerebral cortex
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Jul 01 2014

Thank you for contacting us about this issue. I am tempted to say you are detecting internexin +/- phosphorylation but the bands appear to be too far apart to fit that explanation. Do you have an image with the molecular weight markers, assuming you in...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2012


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit...

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