Anti-alpha Internexin 抗体 [2E3] (ab7654)

製品の概要

  • 製品名
    Anti-alpha Internexin antibody [2E3]
    alpha Internexin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E3] to alpha Internexin
  • 特異性
    specifically recognizes a-internexin.
  • アプリケーション
    適用あり: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mammal
  • 免疫原

    Recombinant full length protein (Rat).

  • エピトープ
    C-terminal non-helical extension of the protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 66 kDa.
Flow Cyt 1/100.
ICC/IF 1/500.
IHC-P 1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • 組織特異性
    Found predominantly in adult CNS.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • 翻訳後修飾
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

画像

  • Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
  • All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)

    Lane 1 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lane 2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lane 3 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lane 4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lane 5 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lane 6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lane 7 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lane 8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lane 9 : Crude homogenate of rat facial nucleus at 28 days after experimental injury
    Lane 10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury


    Predicted band size : 66 kDa
    Observed band size : 66 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab7654 staining alpha Internexin in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA, pH 9.0 (100°C for 20 minutes). Samples were incubated with primary antibody (1/2500) for 20 minutes at 25°C.

    See Abreview

参考文献

This product has been referenced in:
  • Rauch U  et al. Expression of intermediate filament proteins and neuronal markers in the human fetal gut. J Histochem Cytochem 54:39-46 (2006). Read more (PubMed: 16087706) »
  • Uchikado H  et al. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005). Read more (PubMed: 15883343) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 100C, 20 min
Sample
Human Tissue sections (cerebral cortex)
Specification
cerebral cortex
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 01 2014

Thank you for contacting us about this issue. I am tempted to say you are detecting internexin +/- phosphorylation but the bands appear to be too far apart to fit that explanation. Do you have an image with the molecular weight markers, assuming you in...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2012


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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