製品の概要

  • 製品名Anti-alpha Glucosidase II antibody
    alpha Glucosidase II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to alpha Glucosidase II
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide:

    SFQHDPETSVLVLRK

    , corresponding to amino acids 916-930 of Human alpha Glucosidase II, prepared as a multiple antigen peptide (MAP).

  • ポジティブ・コントロール
    • Rat Brain Tissue Extract This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: HeLa and HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IP
  • 追加情報IP: 1/50.
    WB: 1/500 sufficient for detection of a-Glucosidase II in 20µg of rat brain tissue extract by colorimetric analysis.
    It is recommended for Western blot applications, that the secondary antibody be quenched by adding normal sera (of an identical species to secondary host) to the diluent. Detects a band of approximately 110 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
    • 組織特異性Detected in placenta.
    • パスウェイGlycan metabolism; N-glycan metabolism.
    • 配列類似性Belongs to the glycosyl hydrolase 31 family.
    • 細胞内局在Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha glucosidase II alpha subunit antibody
      • Alpha-glucosidase 2 antibody
      • G2AN antibody
      • GANAB antibody
      • GANAB_HUMAN antibody
      • Glu II antibody
      • Glucosidase alpha neutral AB antibody
      • Glucosidase II alpha antibody
      • Glucosidase II subunit alpha antibody
      • GluII antibody
      • KIAA0088 antibody
      • Neutral alpha glucosidase AB antibody
      • Neutral alpha glucosidase AB precursor antibody
      • Neutral alpha-glucosidase AB antibody
      see all

    Anti-alpha Glucosidase II antibody 画像

    • ICC/IF image of ab69489 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69489 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol (100%, 5min) and formaldehyde (4%, 10min) fixed HepG2 cells at 1/200 dilution.

    Anti-alpha Glucosidase II antibody (ab69489) 使用論文

    This product has been referenced in:
    • Iwamoto S  et al. Reconstructed glycan profile for evaluation of operating status of the endoplasmic reticulum glycoprotein quality control. Glycobiology 23:121-31 (2013). Read more (PubMed: 22975980) »
    • Dai L  et al. Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics 11:4021-8 (2011). Read more (PubMed: 21898824) »

    See all 3 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Human Cell (LNCaP)
    Specification LNCaP
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Armen Petrosyan

    Verified customer

    投稿 Nov 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"