製品の概要

  • 製品名Anti-Alpha Fodrin antibody
    Alpha Fodrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Alpha Fodrin
  • 特異性This antibody recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin.
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 162 of mouse alpha-Fodrin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml.

ターゲット情報

  • 機能Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • 関連疾患Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • 配列類似性Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • 翻訳後修飾Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Anti-Alpha Fodrin antibody 画像

  • All lanes : Anti-Alpha Fodrin antibody (ab2558) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 285 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,71 kDa. We are unsure as to the identity of these extra bands.


  • Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa (possible cleavage fragment).

Anti-Alpha Fodrin antibody (ab2558) 使用論文

This product has been referenced in:
  • Woods LT  et al. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303:C790-801 (2012). WB ; Mouse . Read more (PubMed: 22875784) »
  • Grubb DR  et al. Phospholipase C{beta}1b associates with a Shank3 complex at the cardiac sarcolemma. FASEB J 25:1040-7 (2011). WB ; Rat . Read more (PubMed: 21148417) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. I have investigated our records for ab2558 Alpha Fodrin antibody. Regarding mouse reactivity, the answer to this question on the datasheet was provided in 2006. We now have information to confirm that this antibody has bee...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293,HELA and JURKAT)
Loading amount 10 µg
Specification HEK293,HELA and JURKAT
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Sep 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"