製品の概要

  • 製品名Anti-alpha Defensin NP5 antibody [8C8]
  • 製品の詳細
    Mouse monoclonal [8C8] to alpha Defensin NP5
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length alpha Defensin NP5 propeptide. The propeptide is the full length protein. In the mature form, it is cleaved at residue 63 (alanine).

  • ポジティブ・コントロール
    • Human duodenum FFPE tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名8C8
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
  • 組織特異性Paneth cells of the small intestine.
  • 配列類似性Belongs to the alpha-defensin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha 5 antibody
    • Alpha defensin 5 antibody
    • DEF 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • DEFA 5 antibody
    • DEFA5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Defensin-5 antibody
    • Defensin5 antibody
    • HD 5 antibody
    • HD5 antibody
    • HNP 5 antibody
    • HNP5 antibody
    • MGC129728 antibody
    • NP 5 antibody
    • NP5 antibody
    see all

Anti-alpha Defensin NP5 antibody [8C8] 画像

  • IHC image of Defensin NP5 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62757, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-alpha Defensin NP5 antibody [8C8] (ab62757) 使用論文

This product has been referenced in:
  • Stronati L  et al. Altered expression of innate immunity genes in different intestinal sites of children with ulcerative colitis. Dig Liver Dis 42:848-53 (2010). WB ; Human . Read more (PubMed: 20452301) »
  • Arijs I  et al. Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease before and after first infliximab treatment. PLoS One 4:e7984 (2009). IHC-P ; Human . Read more (PubMed: 19956723) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"