製品の概要

  • 製品名Anti-alpha COP I antibody
    alpha COP I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to alpha COP I
  • 特異性Detects coatomer-protein I alpha (COP I alpha).
  • アプリケーション適用あり: IP, ICC, WBmore details
  • 種交差性
    交差種: Rat, Human, Non Human Primates
    交差が予測される動物種: Mouse, Cow, Dog, Rice
  • 免疫原

    Synthetic peptide corresponding to Rat alpha COP I aa 1-19.
    Sequence:

    MLTKFETKSARVKGLSFHP


    (Peptide available as ab4931)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ICC Use a concentration of 3 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100, 140 kDa.Can be blocked with alpha COP I peptide (ab4931).

ターゲット情報

  • 機能The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
    Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
  • 組織特異性Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.
  • 配列類似性Contains 6 WD repeats.
  • 発生段階Xenin is released into the circulation after a meal.
  • 細胞内局在Secreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha coat protein antibody
    • Alpha COP antibody
    • Alpha COPI antibody
    • Alpha-coat protein antibody
    • Alpha-COP antibody
    • AlphaCOP antibody
    • Coatomer protein complex subunit alpha antibody
    • Coatomer subunit alpha antibody
    • COP A antibody
    • COP I alpha antibody
    • copA antibody
    • COPA_HUMAN antibody
    • COPI alpha antibody
    • FLJ26320 antibody
    • HEP COP antibody
    • HEP-COP antibody
    • HEPCOP antibody
    • Proxenin antibody
    • Xenin antibody
    • Xenopsin-related peptide antibody
    see all

Anti-alpha COP I antibody 画像

  • ICC/IF of COP1 in Hela Cells

Anti-alpha COP I antibody (ab2913) 使用論文

This product has been referenced in:
  • Sudo H  et al. Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model. Genomics 95:210-6 (2010). WB ; Human . Read more (PubMed: 20153416) »

See 1 Publication for this product

Product Wall

Thank you for the explanation, and for the image and protocol. The staining you are seeing with the alpha COP anditboy ab2913 does look incorrect, if all the staining is expected to be peri-nuclear, as is seen with the delta COP antibody. The small ...

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Thank you for contacting us. I am sorry to read that you are having trouble getting a good result with this antibody. Can you please tell me what application you are using it for? I am assuming IHC or ICC but I will need a few more details. We alway...

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Thank you for your email. Ab2913 was tested for application in Western blotting using PC-12 cell extract. The antibody detected a band at approximately 140 kDa representing COPI alpha. The antibody also detected a band at approximately 100 kDa, the ide...

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Thank you for submitting the enquiry. The customer is using an inappropriate secondary Ab. The primary Ab is raised in rabbit and so an anti rabbit secondary is necessary not anti goat! If you have any further questions then please do not hesitate to g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"