Anti-alpha + beta Tubulin 抗体 [TU-10] (ab30506)

製品の概要

  • 製品名Anti-alpha + beta Tubulin antibody [TU-10]
  • 製品の詳細
    Mouse monoclonal [TU-10] to alpha + beta Tubulin
  • 特異性ab30506 recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules.
  • アプリケーション適用あり: WB, ICCmore details
  • 種交差性
    Reacts with a wide range of species. Not yet tested in other species.
  • 免疫原

    Microtubule proteins from porcine brain

  • ポジティブ・コントロール
    • Porcine brain lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by precipitation methods and size-exclusion chromatography.
  • ポリ/モノモノクローナル
  • クローン名TU-10
  • アイソタイプIgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab30506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 100 kDa. Use under reducing conditions.
ICC Use at an assay dependent dilution.

ターゲット情報

  • 関連性Tubulin is the major building block of microtubules - an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides alpha and beta. Alpha- and beta-tubulins are encoded by separate genes, or small gene families. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions, the most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes and both undergo different post-translational modifications. Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
  • 細胞内局在Cytoplasmic
  • 別名
    • TUBA1A antibody
    • TUBA3 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2A antibody
    • Tubulin alpha 1a antibody
    • Tubulin beta 2A antibody
    • Tubulin beta antibody
    see all

Anti-alpha + beta Tubulin antibody [TU-10] 画像

  • Anti-alpha + beta Tubulin antibody [TU-10] (ab30506) at 2 µg/ml + Human Jurkat T cell line lysate (1% laurylmaltoside)

    Secondary
    Goat anti-mouse IgG (H+L)-HRP

    Performed under non-reducing conditions.

    Predicted band size : 100 kDa

Anti-alpha + beta Tubulin antibody [TU-10] (ab30506) 使用論文

ab30506 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"