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RabMAb

Anti-alpha + beta Synuclein 抗体 [EP1646Y] (ab51252)

製品の概要

  • 製品名
    Anti-alpha + beta Synuclein antibody [EP1646Y]
    alpha + beta Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1646Y] to alpha + beta Synuclein
  • アプリケーション
    適用あり: Flow Cyt, WB, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human alpha + beta Synuclein aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Human fetal brain, human cerebellum, mouse brain and rat brain tissue lysates. ICC/IF: PC12 and rat primary hippocampal neuron cells. Flow Cyt: SH-SY5Y and U87-MG cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/30.

For unpurified use at 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

For unpurified use at 1/50000.

ICC/IF 1/50 - 1/100.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    Anti-alpha + beta Synuclein antibody [EP1646Y] 画像

    • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling alpha + beta Synuclein with purified ab51252 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Overlay histogram showing U87-MG cells fixed in 4% PFA and stained with purified ab51252 at a dilution of 1 in 30 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
    • All lanes : Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/5000 dilution (purified) + Human cerebellum tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Flow Cytometry analysis of U87-MG cells labelling alpha + beta Synuclein with purified ab51252 at 1/30 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/50000 dilution (unpurified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • Immunocytochemistry/Immunofluorescence analysis of rat primary hippocampal neurons, staining alpha + beta Synuclein with unpurified ab51252. Cells were fixed, permeabilized, and blocked with 10% donkey serum at room temperature. Cells were incubated with primary antibody (1/1000) at 4°C for 24 hours. A Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

    • Overlay histogram showing SH-SY5Y cells stained with unpurified ab51252 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab51252, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) 使用論文

    This product has been referenced in:
    • Binolfi A  et al. Intracellular repair of oxidation-damaged a-synuclein fails to target C-terminal modification sites. Nat Commun 7:10251 (2016). WB, ICC/IF . Read more (PubMed: 26807843) »
    • Woulfe J  et al. Human serum antibodies against EBV latent membrane protein 1 cross-react with a-synuclein. Neurol Neuroimmunol Neuroinflamm 3:e239 (2016). WB ; Human . Read more (PubMed: 27218119) »

    See all 10 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Sample
    Human Cell (SH-SY5Y)
    Specification
    SH-SY5Y
    Permeabilization
    Yes - 0.5% Tween
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Sep 03 2014

    Thank you for your enquiry and for your patience. I can confirm we have the following alpha Synuclein monoclonal antibody which has an N terminal immunogen. ab51252 Rabbit monoclonal [EP1646Y] to alpha Synuclein Reacts with: Mouse, Rat, Human...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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