製品の概要

  • 製品名
    Anti-Alpha B Crystallin (phospho S19) antibody
    Alpha B Crystallin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Alpha B Crystallin (phospho S19)
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 14-25.
    Sequence: FFPFHSpPSRLFD

  • ポジティブ・コントロール
    • Immunofluorescence staining of phospho-alpha-B crystallin (Ser19) in U373 MG cells results in cytoplasmic staining.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.

ターゲット情報

画像

  • All lanes : Anti-Alpha B Crystallin (phospho S19) antibody (ab5597) at 0.5 µg/ml

    Lane 1 : phospho-alpha-B crystallin (Ser19)
    Lane 2 : unphosphorylated alpha-B crystallin

    Observed band size: 20 kDa (why is the actual band size different from the predicted?)



    Western blot of recombinant bovine phospho-alpha-B crystallin (Ser19) using ab5597. Lane 1 is phospho-alpha-B crystallin (Ser19) and lane 2 is unphosphorylated alpha-B crystallin.

参考文献

This product has been referenced in:
  • Lambrecht S  et al. Differential expression of alphaB-crystallin and evidence of its role as a mediator of matrix gene expression in osteoarthritis. Arthritis Rheum 60:179-88 (2009). WB ; Human . Read more (PubMed: 19116904) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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