Anti-Alpha B Crystallin 抗体 [1A7.D5] (ab74441)
Key features and details
- Mouse monoclonal [1A7.D5] to Alpha B Crystallin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Alpha B Crystallin antibody [1A7.D5]
Alpha B Crystallin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1A7.D5] to Alpha B Crystallin -
由来種
Mouse -
特異性
Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Alpha B Crystallin.
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ポジティブ・コントロール
- WB: AlphaB crystallin protein. ICC/IF: SK-N-BE cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
1A7.D5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74441の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Predicted molecular weight: 20 kDa.
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ICC/IF |
1/100.
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特記事項 |
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WB
1/2000. Predicted molecular weight: 20 kDa. |
ICC/IF
1/100. |
ターゲット情報
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機能
May contribute to the transparency and refractive index of the lens. -
組織特異性
Lens as well as other tissues. -
関連疾患
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
配列類似性
Belongs to the small heat shock protein (HSP20) family. -
細胞内局在
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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参照データベース
- Entrez Gene: 1410 Human
- Omim: 123590 Human
- SwissProt: P02511 Human
- Unigene: 53454 Human
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別名
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
画像
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All lanes : Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441) at 1/100 dilution
Lane 1 : AlphaA crystallin protein
Lane 2 : AlphaB crystallin protein
Predicted band size: 20 kDa
Observed band size: 20 kDa -
4% formaldehyde-fixed SK-N-BE cells stained for Alpha B Crystallin (green) using ab74441 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60min room temperature, 5min room temperature. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab74441 (D) Composite.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab74441 は 1 報の論文で使用されています。
- Lomiwes D et al. Small heat shock proteins and toughness in intermediate pHu beef. Meat Sci 95:472-9 (2013). WB ; Cow . PubMed: 23793082