Anti-alpha Adducin (phospho S726) 抗体 (ab53093)

Submit an Abreview Q&A (3)Specific References (1)

製品の概要

  • 製品名
    Anti-alpha Adducin (phospho S726) antibody
    alpha Adducin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to alpha Adducin (phospho S726)
  • 由来種
    Rabbit
  • 特異性
    ab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPSPFL).

  • ポジティブ･コントロール
    • WB: Human brain tissue IHC-P: HeLa cell extract treated with Forskolin This antibody gave a positive result when used in the following formaldehyde fixed cell lines:SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

ターゲット情報

  • 機能
    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • 組織特異性
    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • 配列類似性
    Belongs to the aldolase class II family. Adducin subfamily.
  • ドメイン
    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all

画像

  • Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093)
    Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093)
    labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.
  • Western blot - alpha Adducin (phospho S726) antibody (ab53093)
    Western blot - alpha Adducin (phospho S726) antibody (ab53093)
    All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Forskolin (40nM, 30 mins) with phosphopeptide
    Lane 2 : HeLa cell extract treated with Forskolin (40nM, 30 mins)

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (ab53093)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (ab53093)

    ab53093 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53093 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Tura A  et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). Read more (PubMed: 18757509) »

See 1 Publication for this product

ab53093 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

3 reviews or Q&A

10
Votes: Highest First


thank you for your answer.
However, we think that the interpretation you mentionned does not fit with our results.
Indeed, we have exactly the same blot with ab51130 and ab53093.
We thus suspect that adducin antibody is in fact dire...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab40760.



To check the status of the order pleas...

Read More


thank you for your answer.
However, we think that the interpretation you mentionned does not fit with our results.
Indeed, we have exactly the same blot with ab51130 and ab53093.
We thus suspect that adducin antibody is in fact dire...

Read More

I can send you a replacement vial of an anti-adducin antibody of your choice as we do not stock small sample sizes. So I can process your request, please include your PO number or Abcam order reference number associated with the purchase of ab51130, al...

Read More

Dear Sir, Madame,
I order a number of our antibodies at Abcam, and we are usually pleased by their quality, except for one case.
The following antibodies were obtained:
- - anti alpha-adducin (rabbit polyclonal, ab51130)
- - anti ph...

Read More

Thank you for your enquiry and for providing the attached western blot data.

Here are a few points to consider:

1. Since a small, non-phosphorylated peptide was used as the immunogen for ab51130, itwill not be reactive with total ad...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録