Anti-alpha Adducin (phospho S726) 抗体 (ab53093)
製品の概要
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製品名Anti-alpha Adducin (phospho S726) antibody
alpha Adducin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to alpha Adducin (phospho S726)
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特異性ab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726.
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アプリケーション適用あり: WB, IHC-P, ELISAmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPSPFL).
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ポジティブ･コントロール
- WB: Human brain tissue IHC-P: HeLa cell extract treated with Forskolin This antibody gave a positive result when used in the following formaldehyde fixed cell lines:SKNSH.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab53093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/40000. |
ターゲット情報
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機能Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
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組織特異性Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
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配列類似性Belongs to the aldolase class II family. Adducin subfamily.
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ドメインEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
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細胞内局在Cytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
- Unigene: 183706 Human
see all -
別名
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Anti-alpha Adducin (phospho S726) antibody 画像
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Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093)labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide. -
Western blot - alpha Adducin (phospho S726) antibody (ab53093)All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution
Lane 1 : HeLa cell extract treated with Forskolin (40nM, 30 mins) with phosphopeptide
Lane 2 : HeLa cell extract treated with Forskolin (40nM, 30 mins)
Predicted band size : 81 kDa
Observed band size : 81 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (ab53093)ab53093 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53093 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-alpha Adducin (phospho S726) antibody (ab53093) 使用論文
This product has been referenced in:
- Tura A et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). Read more (PubMed: 18757509) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"